关键词: 猕猴桃, 红肉, 花青素, 查尔酮合成酶, 无色花青素双加氧酶, 基因, 表达

Abstract: Based on the highly conserved sequences of other plants，counterpart sequences of CHS（chalcone synthase，CHS）and LDOX（leucoanthocyanidin dioxygenase，LDOX）of Actinida chinesis，‘Hongyang’were obtained using the special primers. Full-length cDNAs encoding CHS and LDOX were cloned respectively from fruit of‘Hongyang’by RACE（rapid amplification of cDNA ends）. The AcCHS was 1501 bp in length，encoding 389 amino acids. AcLDOX was 1381 bp in length and encoded 355 amino acids. Amino acids sequence of AcCHS exhibited over 95% homology with CHS of Abelmoschus manihot，Camellia japonica and Gossypium hirsutum. It also shared 94% and 93% homology with Vitis vinifera and Malus × domestica respectively. AcLDOX showed 94% and 93% identity with Vitis amurensis  and Vitis vinifera respectively at amino acids level. The expression of AcCHS and AcLDOX in the inner pericarp of three kiwifruit cultivars with red，green and yellow color was analyzed by Real-time PCR. AcCHS expressed highly in flesh of‘Hongyang’at 65 DAF（days after flower）. Expression of the gene in yellow-fleshed‘Jinnong’decreased permanently after flowering. Expression of AcLDOX increased during the early developmental stage in‘Hongyang’，but dropped quickly after 65 DAF. Interestingly，expression of AcLDOX raised markedly in the late developmental stages of green-fleshed‘Jinkui’，which was the highest among three cultivars. AcLDOX in‘Jinnong’also deregulated after flowering.

Key words: kiwifruit, red flesh, anthocyanin, CHS：chalcone synthase, leucoanthocyanidin dioxygenase, gene, expression