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园艺学报 ›› 2012, Vol. 39 ›› Issue (10): 1991-1998.

• 研究报告 • 上一篇    下一篇

‘紫红1 号’红肉苹果果肉抗氧化性及花色苷分析

王燕, 陈学森, 刘大亮, 王传增, 宋杨, 陈晓流, 张艳敏   

  1. (山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安 271018)
  • 出版日期:2012-10-25 发布日期:2012-10-25

Antioxidant Activity and Anthocyanins Analysis of Pulp in ‘Zihong 1’ Red-flesh Apple

 WANG  Yan, CHEN  Xue-Sen, LIU  Da-Liang, WANG  Chuan-Zeng, SONG  Yang, CHEN  Xiao-Liu, ZHANG  Yan-Min   

  1. (State Key Laboratory of Crop Biology,Shandong Agricultural University,Tai’an,Shandong 271018,China)
  • Online:2012-10-25 Published:2012-10-25

摘要: ‘紫红1号’是新疆红肉野苹果[Malus sieversii f. neidzwetzkyana(Dieck)Langenf.]与‘富士’苹果杂交的F1代中的一株果肉全红的株系。以其为试材,测定其果肉的花色苷、多酚、类黄酮含量及其抗氧化能力,结果表明其果肉花色苷含量为228.3 mg · kg-1 FW,总酚含量为2 523 mg · kg-1 FW,类黄酮含量为2 514 mg · kg-1 FW,其抗氧化能力(ferric reducing antioxidant power,FRAP)为13.39 μmol · g-1。利用液质联用(UPLC-PAD-/MS/MS)鉴定了红肉苹果果肉花色苷的主要组分有9种,其中4种分别为矢车菊3–O–葡萄糖苷、矢车菊3–O–半乳糖苷、矢车菊3–O–木糖苷、矢车菊3–O–阿拉伯糖苷,其中矢车菊3–O–半乳糖苷占73.37%,另外5种暂不能确定其结构。本研究表明花色苷的稳定性,发现该色素在pH 1 ~ 3,40 ℃以下比较稳定,高温加速花色苷的降解。花色苷对光照敏感,暴露在室内自然光下15 d其残留率为41.53%。

关键词: 苹果, 红肉, 花色苷, 多酚, 抗氧化, 花色苷稳定性

Abstract: ‘Zihong 1’apple is red-flesh apple,which belonged to F1 of Xinjiang wild red-flesh apple[Malus sieversii f. neidzwetzkyana(Dieck)Langenf.] and‘Fuji’apple. Pulp of‘Zihong 1’apple was taken as material to determine the anthocyanin content,total phenolic content,flavonoid content and antioxidant activity. The results showed that they were 228.3 mg · kg-1 FW,2 523 mg · kg-1 FW,2 514 mg · kg-1 FW and 13.39 μmol · g-1,respectively. Nine anthocyanins were found in red pulp of‘Zihong 1’ apple assayed by UPLC-PAD-/MS/MS,of which four kinds of anthoyanins were detected as cyanidin 3-O-galactoside,cyanidin 3-O-glucoside,cyaniding 3-O-arabinoside and cyanidin 3-O-xyloside. cyanidin 3-O-galactoside was the main anthcyanin,which accounted for 73.3% of total anthocyanins. Structure of the other five anthocyanins couldn’t be determined temporarily. The stability of the anthocyanins was studied and found that the anthocyanins were more stable in aqueous solution which pH value was between 1 and 3,below 40 ℃. The high temperature accelerated degradation of the anthocyanins. The anthocyanins was sensitive to light and the residual amount was only 41.53%.

Key words: apple, red-flesh, anthocyanin, phenolic, antioxidant, anthocyanins stablity

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