关键词: 唐菖蒲, 脂氧合酶, 茉莉酸甲酯, 水杨酸异羟肟酸, 球茎膨大

Abstract: The expression level of GhLOX1 gene was analyzed in the corm of Gladiolus hybridus‘Rose Supreme’by Real time RT-PCR，which were treated by different concentration of MJ（0.1，0.2，0.5 mmol · L^-1）and SHAM（0.5，0.75，1.0 mmol · L^-1）respectively. In addition，LOX activity，endogenesis MJ content，fresh weight and size of corms were also assayed. Analysis results showed that the expression level of GhLOX1 gene，LOX activity and endogenous MJ content，fresh weight and size of corms increased steadily with a MJ gradient from 0.1 mmol · L^-1 to 0.5 mmol · L^-1. Conversely，these indexes steadily decreased with the increasing concentration of SHAM.At the same time，35S-GhLOX1 over expression vector was constructed，and GhLOX1 gene was transformed into the microtuber slices of potato cultivar‘Zhongshu 3’by Agrobacterium tumefaciens-mediated method. The results showed that the number，size and fresh weight of transgenic potato tubers had been increased to varying degrees.

Key words: Gladiolus hybridus, lipoxygenase, methyl jasmonate, salicylhydroxamic acid, corm enlargement