关键词: 梨, 单核苷酸多态性, 功能性标记, 贝壳杉烯氧化酶基因

Abstract: The functional markers based on gene sequences are ideal markers for genotyping，gene mapping and marker assistant selection for associated traits. In this research，5 PCR primer pairs were designed according to the cDNA sequence of PpKO gene in pear which codes the protein of ent-kaurene oxidase（KO），one of the key enzymes in the path of gibberellin synthesis. These primer pairs were tested in the population derived from the cross of‘Aishengli’בChili’by high resolution melting（HRM）ananlysis，and one of them was selected for genotyping. Furthermore，the fine genotyping function of this primer pair was confirmed by 9 cultivars. Sequencing and comparison of amplicons from the progenies and the 9 cultivars showed that the primer pair could amplified a fragment of 200 bp in length，which located in the eighth exon of PpKO gene，with 2 non-synonymous SNPs（single nucleotide polymor- phisms）detected. The two SNPs are genetic markers suitable for high resolution analysis，which are useful tools for genetic mapping，gene location and germplasm identification.

Key words: pear, single nucleotide polymorphism, functional marker, ent-kaurene oxidase gene