关键词: 结球甘蓝, 花粉, 膜联蛋白

Abstract: Plant annexins constitute a multigene family having suggested roles in a variety of cellular processes. We have isolated and characterized an annexin cDNA of cabbage（Brassica oleracea L. var. capitata L.）gene（BoAnnexin2）encoding annexin proteins using RT-PCR and RACE-PCR after 2-D electrophoresis of cabbage pollen total proteins，which found that annexin protein was downregulated when pollens were germinated. The BoAnnexin2 gene was 1 157 bps，which contained 951 bps open reading frame. The predicted molecular mass of BoAnnexin2 is 36.02 kD with acidic pI of 6.33. At the amino acid level，BoAnnexin2 contains two typeⅡCa2+ binding domain repeats，there was a GXXXGXS （T）/DXXG motif in the fourth domain. The quantitative RT-PCR revealed that the BoAnnexin2 gene expression in mature pollen is eight times higher than that in germinated pollen. These results indicate that BoAnnexin2 gene may play an important role in cabbage pollen germination.

Key words: cabbage, pollen, annexin