早熟苹果花青苷积累与其相关酶活性及乙烯生成之间的关系

园艺学报 ›› 2012, Vol. 39 ›› Issue (7): 1235-.

早熟苹果花青苷积累与其相关酶活性及乙烯生成之间的关系

刘金, 魏景立, 刘美艳, 宋杨, 冯守千, 王传增, 陈学森

山东农业大学作物生物学国家重点实验室，山东泰安 271018；山东省冠县国有毛白杨林场，山东冠县 252500

出版日期:2012-07-25 发布日期:2012-07-25

The Relationships Between the Enzyme Activity of Anthocyanin Biosynthesis，Ethylene Release and Anthocyanin Accumulation in Fruits of Precocious Apple Cultivars

LIU Jin, WEI Jing-Li, LIU Mei-Yan, SONG Yang, FENG Shou-Qian, WANG Chuan-Zeng, CHEN Xue-Sen

State Key Laboratory of Crop Biology，Shandong Agriculture University，Tai’an，Shandong 271018，China；Guanxian
State Forest of Populas Tomentosa，Guanxian，Shandong 252500，China

Online:2012-07-25 Published:2012-07-25

摘要/Abstract

摘要： 以生长发育期相近但着色不同的早熟苹果‘泰山早霞’（红色）和‘辽伏’（绿色）为试材，研究果实生长发育过程中果皮花青苷含量和PAL、CHI、UFGT 活性，以及乙烯释放速率的变化。结果显示：①在果实发育过程中，‘泰山早霞’花青苷含量与苯丙氨酸解氨酶（PAL）、查尔酮异构酶（CHI）、类黄酮半乳糖苷转移酶（UFGT）活性均明显高于‘辽伏’。②两个早熟苹果在采收前均有乙烯释放高峰，并且乙烯释放高峰早于花青苷的迅速积累；喷施1-MCP 后果实的乙烯释放速率降低，花青苷合成随之显著减少：说明乙烯启动并调控早熟苹果成熟期花青苷的积累。③‘泰山早霞’发育后期花青苷含量与UFGT活性显著正相关，乙烯可能是通过调控UFGT 酶的活性来促进花青苷的合成。

关键词: 苹果, 早熟, 花青苷, 苯丙氨酸解氨酶, 查尔酮异构酶, 类黄酮半乳糖苷转移酶

Abstract: Fruits of precocious red‘Taishan Zaoxia’and green‘Liaofu’apple cultivars demand for the same growth period before ripening were investigated. The results indicated that：①The content of anthocyanin and the activities of PAL，CHI and UFGT in‘Taishan Zaoxia’were higher than those in ‘Liaofu’（P < 0.05）. ②The release of ethylene in both cultivars peaked before harvesting and the rapid accumulation of anthocyanin. The ethylene release of the fruit reduced after the application of 1-MCP，and followed by the significant decrease of the anthocyanin release；The ethylene promoted and affected the accumulation of anthocyanin at the ripening stage of precocious apples. ③The content of anthocyanin was positively correlated with the activities of UFGT in‘Taishan Zaoxia’during fruits ripening. The production of ethylene is closely related to the synthesis of anthocyanin by mediating the activity of UFGT.

Key words: apple, precocious, anthocyanin, phenylalanine ammonia-lyase, chalcone isomerase, glycosyltransferase

中图分类号:

S 661.1

刘金, 魏景立, 刘美艳, 宋杨, 冯守千, 王传增, 陈学森. 早熟苹果花青苷积累与其相关酶活性及乙烯生成之间的关系[J]. 园艺学报, 2012, 39(7): 1235-.

LIU Jin, WEI Jing-Li, LIU Mei-Yan, SONG Yang, FENG Shou-Qian, WANG Chuan-Zeng, CHEN Xue-Sen. The Relationships Between the Enzyme Activity of Anthocyanin Biosynthesis，Ethylene Release and Anthocyanin Accumulation in Fruits of Precocious Apple Cultivars[J]. ACTA HORTICULTURAE SINICA, 2012, 39(7): 1235-.

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