关键词: 钙调素, 自交不亲和性, 自发荧光, 激光共聚焦显微镜

Abstract: Confocal laser scanning microscopy (CLSM)were used to investigate the role of exogenous calmodulin (CaM) and anti-CaM serum in 'Hosui' style auto-fluorescence characterization after self- and cross-pollination for 12 h and 72 h. The results showed that the auto-fluorescence of cross-pollination topside style treated with CaM for 12h reduced and the bottom increased, but the auto-fluorescence of self-pollination style treated with CaM increased except 2 500～3 000 μm behind stigma. Anti-CaM serum treated cross-pollination style increased the whole auto-fluorescence except 2 500～3 000 μm behind stigmaand the tiptop of self-pollination after anti-CaM serum treatment moved to 1 000～1 500 μm behide stigma. After pollination 72 h, the style of cross-pollination treated with CaM increased its auto-fluorescence and the highest value was at ovary. Self-pollination style treated with CaM changed the distributing disciplinarian of auto-fluorescence, its tiptop were at 1 500～2 000 μm behide stigma.The highest auto-fluorescence of self- and cross-pollination treated with anti-CaM serum moved to 0～500 μm behide stigma. This primary evdience showed here confirmed that exogenous calmodulin and anti-CaM serum have effected on metabolize of the self- and cross-pollination.

Key words: calmodulin, self-incompatible, auto-fluorescence, confocal laser scanning microscopy