关键词: 百合大花卷丹, 百合尖孢镰刀菌, SSH 文库, 基因表达分析

Abstract: Tissue culture seedlings of 20 lily germplasms were identified for resistance against Fusarium oxysporium by an in vitro inoculation method in laboratory，the highly resistant lily（Lilium leichtlinii var. maximowivzii Baker）was used to construct the forward suppression subtractive hybridization（SSH）library and the backward SSH library using the SMART method. The seedlings were induced by F. oxysporum f. sp. lilii for 12 h and 24 h after inoculation and those treated by water were taken as control. Four resistance-related ESTs were analyzed by the semi-quantitative RT-PCR. The results showed that 3 of 20 identified lily germplasms were highly resistant，nine of them were middle resistant and 8 were susceptible；The inserted segments of SSH library were 200–2 000 bp in length；According to the results of dot blot hybridization，fiftyclones of the forward library were picked out and sequenced，and then 6 classes 10 resistance-related ESTs were obtained. The RT-PCR results of 4 resistance-related genes （serine/threonine protein kinase，glutathione S-transferase，peroxidase，cyclophilin etc.）showed that they were upregulated by F. oxysporum f. sp. lilii and supposed to involved in the disease resistance reaction for Fusarium wilt.

Key words: Lilium leichtlinii var. maximowivzii Baker, Fusarium oxysporum f. sp. lilii, suppression subtractive hybridization（SSH）library, gene expression analysis