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园艺学报 ›› 2012, Vol. 39 ›› Issue (6): 1081-1089.

• 蔬菜 • 上一篇    下一篇

青花菜相同亲本的DH 与F2 群体遗传多样性的比较

孙继峰, 刘玉梅, 方智远, 刘二艳, 袁素霞, 李占省, 杨丽梅, 庄木, 张扬勇, 孙培田   

  1. (中国农业科学院蔬菜花卉研究所,北京 100081)
  • 出版日期:2012-06-25 发布日期:2012-06-25

The Comparison Analysis of Genetic Diversity Between DH Population and F2 Population Derived from the Same Two Parents in Brassica oleracea var.
italica

SUN  Ji-Feng, LIU  Yu-Mei, FANG  Zhi-Yuan, LIU  二Yan, YUAN  Su-Xia, LI  Zhan-Sheng, YANG  Li-Mei, ZHUANG  Mu, ZHANG  Yang-Yong, SUN  Pei-Tian   

  1. (Institute of Vegetable and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
  • Online:2012-06-25 Published:2012-06-25

摘要: 以表型差异明显的两个青花菜(Brassica oleracea var. italica)高代自交系为材料杂交获得F1,通过游离小孢子培养技术和人工自交方法,分别构建了由176个DH系组成的DH群体和由176个单株组成的F2群体。采用性状分离分析和群体遗传多样性分析方法,对两个群体13个主要农艺性状的平均值、变异系数及性状分离区间等遗传表现进行分析。结果表明:13个主要农艺性状均是受多基因控制的数量性状,在DH群体和F2群体中均发现了正向和负向两个方向的超亲基因型,两群体的平均变异系数和遗传多样性指数相近。可见,通过DH 技术育种不仅可以加快亲本的纯化进程,而且可获得广泛分离的育种资源,从而提高育种效率。

关键词: 青花菜, DH 群体, F2 群体, 性状分离, 遗传多样性

Abstract: By isolated microspore culture techniques and artificial self-crossing method,DH population with 176 lines separately and F2 population with 176 individuals were derived from a broccoli combination of two high-generation self-bred lines which were obviously different in phenotype. Average value,coefficient of variation(CV)and segregation region of 13 main agronomic characters were studied by analyzing the separation of agronomic characters and the genetic diversity. The 13 main agronomic traitswere generally controlled by multiple genes and inherited as quantitative trait loci(QTLs). The positive and negative transgress genotypes could be generated in DH population and F2 population.The average CV and genetic diversity index were similar between DH population and F2 population.Therefore,the DH population could be used as breeding material to obtain breeding resources with wider segregation of character and accelerated the progress of parental purification and enhanced the breeding efficiency.

Key words: broccoli, DH population, F2 population, character segregation, genetic diversity

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