https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
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https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
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https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2012, Vol. 39 ›› Issue (6): 1035-1044.

• 果树 • 上一篇    下一篇

桃自交亲和性的分子机制及遗传特性研究

 许高歌, 吴华清, 吴俊, 王超, 齐开杰, 张绍铃   

  1. 南京农业大学园艺学院,南京 210095
  • 出版日期:2012-06-25 发布日期:2012-06-25

Molecular Mechanism and Genetic Characterization of Self-compatibility in Peach

 XU  Gao-Ge, WU  Hua-Qing, WU  Jun, WANG  Chao, QI  Kai-Jie, ZHANG  Shao-Ling   

  1. College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China
  • Online:2012-06-25 Published:2012-06-25

摘要: 以油桃(Prunus persicavar.necturina)‘中油5号’及其自交后代为试材,利用花柱S-RNase基因和花粉SFB基因的特异性引物进行PCR扩增,对扩增片段进行回收、克隆、测序和Blast分析,确定了‘中油5号’的自交不亲和基因型为S1S2(m)。序列分析结果显示,‘中油5号’桃S1-RNaseS2(m)-RNase与多个其他李属S-RNase基因氨基酸和核苷酸序列间都具有极高的相似性,SFB1SFB2基因与多个其他李属SFB基因氨基酸和核苷酸序列间也都具有极高的相似性。进一步对‘中油5号’自交后代个体的S基因型进行检测,发现后代群体中存在3种S基因型,分别为S1S1S1S2(m)S2(m)S2(m),且分离比为8137,与预期的比例121差异不显著(χ2 = 0.214 < χ20.05,2 = 5.991),说明S-RNase基因和SFB基因都遵循两因素的孟德尔遗传规律进行连锁分离。此外,基因型为S1S1S2(m)S2(m)的纯合体的存在,表明桃花粉SFB1SFB2基因均不能被自身花柱S-RNase所识别,这是由于SFB1SFB2基因翻译提前终止所造成的,从而使‘中油5号’表现出自交亲和性。

关键词: 桃, 花柱S-RNase 基因, 花粉SFB 基因, 自交亲和

Abstract: PCR were performed with specific primer sets of Prunus S-RNase and SFB alleles using ‘Zhongyou 5’(Prunus persica var.necturina)as the template. The amplification products were extracted,cloned,sequenced and then Blast analysis,which were used to determine the S-genotype of this cultivar,S1S2(m). Sequence analysis showed that Prunus persica S1-RNase and S2(m)-RNase alleles have exceptionally high identities with other Prunus S-RNase alleles,and,SFB1 and SFB2 alleles have exceptionally high identities with other Prunus SFB alleles,too. The S-genotypes of self-pollinated progeny of‘Zhongyou 5’were detected,it is obviously that three classes were found in progeny,and the segregation ratio of S1S1S1S2(m) and S2(m)S2(m) was 8137,which did fit to the expected ratio that was 121(χ2 = 0.214 < χ20.05,2 = 5.991). This result showed that S-RNase and SFB alleles are tightly co-segregation according to the Mendel genetic model. Moreover,the existence of S1S1 and S2(m)S2(m) homozygotes suggested that SFB1 and SFB2 could not be cognized by self S-RNase,due to that the protein of these two SFB alleles were premature,which resulted in the‘Zhongyou 5’is self-compatible.

Key words: peach, S-RNase, SFB, self-compatibility

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