板栗采后次氯酸钠水浴处理时间对冷藏期间几种酶活性的影响

园艺学报 ›› 2008, Vol. 35 ›› Issue (11): 1595-1600.

板栗采后次氯酸钠水浴处理时间对冷藏期间几种酶活性的影响

王贵禧^1*,杨小胡²,梁丽松¹,石雪晖³

（¹中国林业科学研究院林业研究所，国家林业局林木培育实验室，北京100091；²湖南省林业科学院，长沙410004；³湖南农业大学园艺园林学院，长沙 410128）

收稿日期:2008-08-04 修回日期:2008-10-11 出版日期:2008-11-25 发布日期:2008-11-25
通讯作者: 王贵禧

Effects of Postharvest NaOCl Water-curing Treatment on Several Enzyme Activities of Chinese Chestnut（Castanea mollissima Blume）During Cold Storage

WANG Gui-xi^1*;YANG Xiao-hu²;LIANG Li-song¹;and SHI Xue-hui³

(¹Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Forest Silviculture of State Forestry Administration，Beijing 100091, China; ²Hunan Forestry Academy, Changsha 410004, China; ³Horticulture and Landscape College, Hunan Agriculture University, Changsha 410128 ,China)

Received:2008-08-04 Revised:2008-10-11 Online:2008-11-25 Published:2008-11-25
Contact: WANG Gui-xi

摘要/Abstract

摘要： 以‘燕昌’板栗为试材，研究了采后NaOCl（500 mg·L-1）水浴处理时间（2 d、3 d、4 d和5 d）对其在0℃冷藏期间几种酶活性的影响。结果表明：水浴2 d处理的淀粉酶活性与对照差异不显著，而水浴4d和5 d处理可显著抑制淀粉酶活性。不同时间NaOCl水浴处理对PPO活性均有一定的抑制作用，但以水浴4 d和5 d处理效果较好。4 d和5 d水浴处理有利于保持POD的活性。水浴处理有效保持了板栗在贮藏期间的SOD活性，水浴3 d、4 d和5 d处理贮藏60 d时表现出活性高峰，且在贮藏期间与其他处理和对照差异显著。NaOCl水浴处理有利于保持CAT在板栗贮藏过程中活性， 4 d和5 d水浴处理的ADH活性总体高于其他处理和对照。总体来说，水浴4 d 和5 d处理对抑制板栗的淀粉酶、PPO活性和提高POD、CAT、SOD和ADH活性有一定效果，从而有利于延缓板栗在贮藏期间的衰老。

关键词: 板栗, NaOCl, 水浴, 冷藏, 酶活性

Abstract:

Different postharvest NaOCl（500 mg·L-1）water-curing treatment durations (2 d, 3 d, 4 d and 5d) were conducted on Chinese chestnut (Castanea mollissima Blume 'Yanchang') and the changes of several enzyme activities (amylase, PPO, POD, SOD, CAT and ADH) were monitored during storage at 0℃. The results revealed that the amylase activity was not affected by 2 d water-curing treatment, while 4 d and 5 d water-curing treatments could keep amylase activity at a lower level than control. All the treatments reduced PPO activities, and the most effective durations were 4 d and 5 d. Water-curing durations of 4 d and 5 d increased the POD activities during the cold storage. All the treatments enhanced the SOD activity, among which 3 d, 4 d and 5 d had the better results than the other treatment and control. CAT activity was enhanced by water-curing at all storage period, and for ADH, 4d and 5d treatments were ideal. This experiment indicated that the pre-storage NaOCl water-curing treatments durations of 4 d and 5 d were the most benefit for chestnut storage.

Key words: chestnut, NaOCl, water-curing, cold storage, enzyme activities

中图分类号:

S 664.2

王贵禧;杨小胡;梁丽松;石雪晖. 板栗采后次氯酸钠水浴处理时间对冷藏期间几种酶活性的影响[J]. 园艺学报, 2008, 35(11): 1595-1600.

WANG Gui-xi;YANG Xiao-hu;LIANG Li-song;and SHI Xue-hui . Effects of Postharvest NaOCl Water-curing Treatment on Several Enzyme Activities of Chinese Chestnut（Castanea mollissima Blume）During Cold Storage[J]. ACTA HORTICULTURAE SINICA, 2008, 35(11): 1595-1600.

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