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园艺学报 ›› 2012, Vol. 39 ›› Issue (5): 977-984.

• 研究报告 • 上一篇    下一篇

一个控制芜菁肉质根直径的遗传新位点 frs-1

张明科, 姚远颋, 钟蔚丽, 何玉科   

  1. (1 西北农林科技大学园艺学院,陕西杨凌 712100;2 中国科学院上海生命科学研究院植物生理生态研究所,植物分子遗传国家重点实验室,上海 200032)
  • 出版日期:2012-05-25 发布日期:2012-05-25

A Novel Locus,frs-1,That Controls Flesh Root Diameter of Turnip

 ZHANG  Ming-Ke, YAO  Yuan-Ting, ZHONG  Wei-Li, HE  Yu-Ke   

  1. (1College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China;2National Key Laboratory of Plant Molecular Genetics,Shanghai Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China)
  • Online:2012-05-25 Published:2012-05-25

摘要: 于春秋两季种植大白菜与芜菁自交系杂交产生的F2群体,筛选与控制芜菁(Brassica campestris L. ssp. rapifera Matzg)肉质根直径基因紧密连锁的分子标记,检测控制肉质根直径的QTL数量、效应及其在图谱中的位置。试验结果表明:F2群体的肉质根直径大小偏向于芜菁,且呈连续性分布,表明该性状为数量性状,受多个基因控制。在春季F2群体中共检测到4个QTLs,分别位于A09、A07、A02和A03连锁群。在秋季群体中采用BSA法筛选到23个与控制肉质根直径基因连锁的标记,构建出A09连锁群片段,在其内检测到1个QTL位点(flesh root size 1),LOD 值为8.19,贡献率为36.07%。该遗传位点在春秋两季群体中检测稳定,是一个新的主效QTL,可在今后分子标记辅助选择育种及基因克隆中加以利用。

关键词: 芜菁, 肉质根, 直径, QTL

Abstract: To screen molecular markers linked to genes tightly controlling flesh root diameter of turnip(Brassica campestris L. ssp. rapifera Matzg)and to identify the QTL number,effect and location in the map,two F2 populations from the cross between Chinese cabbageBre-1-1-1-1and turnip W-2-1-8-1 were studied in spring and autumn. The flesh root diameters of F2 populations were changed continuously and marked bias towards the parent turnip,suggesting that it was quantitative trait,controlled by multiple genes. Four QTLs for controlling flesh root diameter were screened in spring F2 population,mapped on A09,A07,A02 and A03,respectively. A QTL locus frs-1 was detected on partial linkage group A09,which was constructed with 23 markers screened by bulked segregation analysis(BSA)in autumn F2 population,the contribution of frs-1 to the total variation was 36.07% with LOD score of 8.19. This locus was a novel and major QTL,stable detection across the two seasons and useful for marker-assisted selection and for gene cloning of flesh root diameter.

Key words: turnip, flesh root, diameter, QTL