https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2012, Vol. 39 ›› Issue (5): 970-976.

• 研究报告 • 上一篇    下一篇

阿月浑子与黄连木的杂种果实发育及幼胚培养研究

庞曼, 李旭新, 董倩, 王洁, 白志英, 路丙社   

  1. (1 河北农业大学林学院,河北保定 071000;2 河北省林木种质资源与森林保护重点实验室,河北保定 071000;3 河北农业大学园林与旅游学院,河北保定 071000;4 河北农业大学生命科学学院,河北保定 071000)
  • 出版日期:2012-05-25 发布日期:2012-05-25

Studies on Fruit Growth Pattern and Immature Embryo Culture of Hybrids Between Pistacia vera and Pistacia chinensis

 PANG  Man, LI  Xu-Xin, DONG  Qian, WANG  Jie, BAI  Zhi-Ying, LU  Bing-She   

  1. (1College of Forestry,Agricultural University of Hebei,Baoding,Hebei 071000,China;2The Key Laboratory of Germplasm Resources of Forest Trees and Forests Protection,Baoding,Hebei 071000,China;3College of Landscape and Tourism,Agricultural University of Hebei,Baoding,Hebei 071000,China; 4 College of Life Science,Agricultural University of Hebei,Baoding,Hebei 071000,China)
  • Online:2012-05-25 Published:2012-05-25

摘要: 以阿月浑子与黄连木的杂种果实和杂种幼胚为试材,对果实生长发育规律及幼胚培养进行了研究,探讨了胚龄、培养基类型和6-BA浓度等对杂种胚培苗再生的影响。结果表明:(1)杂种果实的纵、横径生长呈“S”型曲线变化,果实鲜质量生长呈双“S”型曲线变化。(2)杂种胚的发育始于授粉后80 d,80 ~ 100 d是杂种胚败育的关键时期。(3)授粉后80 d的杂种幼胚难以成苗,授粉后100和120 d的杂种幼胚成苗率分别为37.04%和83.33%。(4)适宜杂种胚培苗增殖的培养基为:DKW + 2.0 mg · L-1 6-BA + 0.05 g · L-1 IBA + 0.3 g · L-1 LH + 1.5 g · L-1 PVP,增殖系数为3.47,苗高为3.32 cm。(5)适宜杂种胚培苗生根的培养基为:1/2WPM + 2.0 mg · L-1 IBA + 0.05 mg · L-1 NAA,生根率为80%。

关键词: 阿月浑子, 杂种, 胚培养

Abstract: Fruits and immature embryos sampled from hybrids between Pistacia vera and Pistacia chinensis were used to study the fruit growth pattern and the effects of different embryo developmental stages,the varieties of medium and concentrations of 6-BA in culture medium on hybrid plants regeneration. The results showed that:(1)The hybrid fruit growth had a single-peak curve pattern in terms of the changing of fruit length and diameter during fruit development,and had a double“S”curve in terms of the changing of fresh weight.(2)The development of hybrid embryo began from 80 days after pollination,and the critical period for the hybrid embryo abortion was from the 80 to 100 days.(3)The hybridimmature embryos collected at 80 days after pollinationdid not germinated while that collected at 100 days and 120 days germinated at rates of 37.04% and 83.33% respectively.(4)The suitable multiplication medium for immature embryo seedlings was DKW + 2.0 mg · L-1 6-BA + 0.05 mg · L-1 IBA + 0.3 g · L-1 LH + 1.5 g · L-1 PVP,with which the multiplication coefficient of 3.47 and the seedling height of 3.32 cm were obtained.(5)The suitable rooting medium was 1/2 WPM + 2.0 mg · L-1 IBA + 0.05 mg · L-1 NAA with a rooting rate of 80%.

Key words: pistachio, hybrid, embryo culture