https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2012, Vol. 39 ›› Issue (5): 931-938.

• 观赏植物 • 上一篇    下一篇

野鸢尾和射干属间杂交亲和性及杂种鉴定

 毕晓颖, 李卉, 娄琦, 郑洋   

  1. (1 沈阳农业大学园艺学院,沈阳 110866;2 辽宁省北方园林植物与地域景观高校重点实验室,沈阳 110866)
  • 出版日期:2012-05-25 发布日期:2012-05-25

Studies on Inter-generic Compatibility of Iris dichotoma and Belamcanda chinensis and Their Hybrids Identification

 BI  Xiao-Ying, LI  Hui, LOU  Qi, ZHENG  Yang   

  1. (1College of Horticulture,Shenyang Agricultural University,Shenyang 110866,China;2Key Laboratory of Northern Landscape Plants and Regional Landscape,Shenyang 110866,China)
  • Online:2012-05-25 Published:2012-05-25

摘要: 以鸢尾科鸢尾属的野鸢尾(Iris dichotoma)和射干属的射干(Belamcanda chinensis)为试材,对其常规授粉后的结实性、花粉管行为及胚胎发育过程进行了观察,并对其杂种进行了形态学和根尖细胞染色体鉴定。结果表明:野鸢尾和射干属间杂交存在一定程度障碍,但仍具有良好的亲和性,且正、反交存在差异。以野鸢尾为母本与射干杂交时,部分花粉不萌发,或者花粉管在柱头或花粉粒上缠绕;不亲和障碍主要发生在受精前,结实率和有胚率分别为48.1%和69.3%。以射干为母本与野鸢尾杂交时,在珠孔端出现胚的解体现象,不亲和障碍主要发生在受精后,结实率和有胚率分别为87.9%和54.3%。所得杂种F1的株高、花冠幅、分蘖能力都介于野鸢尾和射干之间;杂种的花期和果期较亲本有所延长,叶宽大于亲本;根状茎、花形与野鸢尾相似;花色、雄蕊、花柱、果实和种子与亲本迥异。杂种根尖细胞染色体数与亲本相同,2n = 2x = 32,均为1B型,杂交后代的染色体数相对保守,变异较小,且核型具有较高的对称性。

关键词: 野鸢尾, 射干, 远缘杂交, 亲和性, 杂种鉴定

Abstract: The cross-compatibility between Iris dichotoma and Belamcanda chinensis was studied by capsule-setting,pollen tube behaviors and embryo development after common pollination,and their F1 hybrids were identified by morphology and cytology. The results showed that the compatibility between I. dichotoma and B. chinensis was good,although there were barriers to a certain degree. The pre-fertilization barriers,when I. dichotoma acted as female parent,was due to the fact that some of the pollen tubes winded the stigma and pollen grains or the pollen grains didn’t germinate,and the capsule setting rate and embryo rate were 48.1% and 69.3%,respectively. The post-fertilization barriers,when B. chinensis acted as female parent,was due to the fact that the embryo disintegrate from micropyle,and the capsule setting rate and embryo rate were 87.9% and 54.3%,respectively. The height,corolla and tiller of the F1 hybrids were all between their parents. The flowering stage and fruit-setting stage were longer and the leaves were wider than their parents;The rootstalk and flower pattern were similar to I. dichotoma. The color of flowers,the stamen,the style,the fructification and the seeds were quite different from their parents. The chromosome numbers of F1 and their parents were all 2n = 2x = 32,and the karyotype types were all 1B,the hybrids were conservative in chromosome number and karyotype type comparing with their parents.

Key words: Iris dichotoma, Belamcanda chinensis, intergeneric hybridization, compatibility, hybrid identification