关键词: 葡萄, 水杨酸, 茉莉酸, 克隆, 表达

Abstract: To understand gene response to exogenous signals in SA and JA signal transduction pathways of grapevine and analysis of the cross-interaction between these two pathways，this study cloned the genes of NPR1，PR1，COI1 and LOX2 from grapevine‘Fujiminori’（Vitis vinifera × Vitis labrusca）. The identity analysis of them with their homologous genes in different plants indicated that the related genes in SA and JA signaling pathways were conserved at relatively high level. Quantitative and semi-quantitative PCR were employed to analyze the expression of the four genes at different levels of hormone treatment，and the results showed that PR1 was specifically induced by the SA，and inhibited after JA applied；SA inhibited expression of LOX2（a gene specifically induced by JA）. According to expression of the two pairs of upstream and downstream genes belonging to SA and JA signalingpathways，the genes PR1 and LOX2 could be considered as the marker genes of these two signal transduction pathways in grapevine. The expression levels of NPR1，PR1，COI1 and LOX2 increased rapidly during the 6–24 h after treatment，and the antagonistic effect between SA and JA pathways may also exist in grapevine.The interactions between SA and JA pathways could be affected by the relative concentration of each hormone.

Key words: grapevine, SA, JA, clone, expression analysis