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园艺学报 ›› 2012, Vol. 39 ›› Issue (4): 705-712.

• 蔬菜 • 上一篇    下一篇

辣椒乙烯反应转录因子基因CaJERF1 的克隆及诱导表达

 张秋平, 杨宇红, 茆振川, 陈国华, 谢丙炎   

  1. (1 中国农业科学院蔬菜花卉研究所,北京 100081;2 湖南农业大学,长沙 410128)
  • 出版日期:2012-04-25 发布日期:2012-04-25

Cloning and Inducible Expression of Ethylene Response Factor CaJERF1 in Hot Pepper

ZHANG  Qiu-Ping, YANG  Yu-Hong, MAO  Zhen-Chuan, CHEN  Guo-Hua, XIE  Bing-Yan   

  1. (1Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2Hunan Agricultural University,Changsha 410128,China)
  • Online:2012-04-25 Published:2012-04-25

摘要: 应用RT-PCR、RACE技术从乙烯诱导的辣椒中克隆到一个乙烯反应(ERF)类转录因子基因,命名为CaJERF1,其全长cDNA序列为1 379 bp,编码375个氨基酸,分子量为41.5 kD,具有一个由59个氨基酸构成的保守的ERF结构域。CaJERF1的AP2域与CaPF的同源性为100%,与JERF1的同源性为99%;全序列与CaPF和JERF1的同源性也分别达到99%和86%,属于ERF家族的第Ⅳ次家族。诱导分析表达结果表明,植物激素乙烯、苿莉酸甲酯以及盐和低温胁迫均可诱导该基因的表达,表明CaJERF1可能参与到多条信号途径中,并在多种逆境胁迫应答中发挥作用。

关键词: 辣椒, ERF, 转录因子, 乙烯, 植物激素, 诱导表达

Abstract: An ethylene response factor gene CaJERF1 was isolated from hot pepper using the methods of RT-PCR and RACE. The full length of the cDNA sequence is 1 379 bp,encoding a protein of 375 amino acids,with predicted molecular weight 41.5 kD. The putative protein contains a conserved ERF motif of 59 amino acids that shares 100% and 99% identities with this domain of CaPF and tomato JERF1,respectively. Alignment of full sequences indicates that CaJERF1 protein has high similarity to CaPF(99%)and JERF1(86%),belonging to Ⅳ subgroup of ERF subfamily. Northern blot analyses showed that ethylene,jasmonic acid,high salt and low temperature obviously induced the expression of CaJERF1,suggesting that CaJERF1 might be involved in multiple stress responses.

Key words: pepper, ethylene response factor, transcription factor, ethylene, plant hormones, inducible expression

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