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园艺学报 ›› 2012, Vol. 39 ›› Issue (4): 661-668.

• 蔬菜 • 上一篇    下一篇

控制大白菜和白菜型油菜叶缘裂刻的QTL 定位及分析

 邓杰, 王辉, 程锋, 武剑, 王晓武   

  1. (中国农业科学院蔬菜花卉研究所,北京 100081)
  • 出版日期:2012-04-25 发布日期:2012-04-25

QTL Mapping of a Leaf-lobed Trait in Brassica campestris

 DENG  Jie, WANG  Hui, CHENG  Feng, WU  Jian, WANG  Xiao-Wu   

  1. (Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

  • Online:2012-04-25 Published:2012-04-25

摘要: 利用叶缘光滑无裂刻的大白菜[Brassica campestris L. ssp. pekinensis(Lour)Olsson]材料‘Z16’和叶缘深裂的白菜型油菜(Brassica campestris L. var. yellow sarson Prain)‘Yellow Sarson 143’构建的120 个株系的BC2DH 群体及已构建的包含10 个连锁群的遗传图谱,利用JoinMap4 软件及MQM 作图法,对叶缘裂刻性状进行QTL 定位分析。3 次重复试验中,分别在第3、10 号染色体上的相同位置各检测到1 个控制叶缘裂刻性状的QTL,具有重复性。各QTL的LOD值在7.66 ~ 18.99 间,可解释26.4% ~ 44.7%的表型变异。通过大白菜与拟南芥注释基因比对,生物活性赤霉素(GA1、GA4)合成的关键基因BrGA20ox3位于第10 染色体的QTL 区域,对143 施加外源赤霉素GA3 能明显抑制叶缘裂刻表型,因此BrGA20ox3为控制叶片裂刻的可能候选基因。对BrGA20ox3 克隆测序获得与该基因共分离的特异标记(BrIDlobe-2),对进一步研究叶缘裂刻性状及分子标记辅助育种具有重要的意义。

关键词: 大白菜, 白菜, 裂刻, QTL, BrGA20ox3

Abstract: The objective of this study was to map and analyze QTLs controlling leaf-lobed trait in the population constructed by Chinese cabbage ‘Z16’ [Brassica campestris L. ssp. pekinensis(Lour)Olsson] and ‘Yellow Sarson 143’ (Brassica campestris L. var. yellow sarson Prain),so as to provide information
for molecular marker assisted selection in Chinese cabbage breeding. Leaf-lobed trait was detected in parents and individuals of BC2DH population of Z16 × 143 in different growing seasons. An integrated map of 559.08 cM with 10 linkage groups was also used for the QTL analysis. Using the genetic map and
multiple QTL model(MQM)method of software package MapQTL(version 4.0),QTLs were detected in the same position on 3rd and 10th chromosomes in the three different growing seasons. The LOD values varied from 7.66 to 18.99 which explained 26.4% - 44.7% of the phenotypic variation. When analyzed with annotated genes between Arabidopsis thaliana and B. rapa in the QTL region on 10th chromosome,one active GA biosynthetic gene was found as the candidate gene for the leaf-lobed trait. The lobed phenotype was inhibited with application of exogenous GA3 in line 143.

Key words: Chinese cabbage, Brassica campestris, leaf-lobed trait, quantitative trait locus(QTL), BrGA20ox3

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