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园艺学报 ›› 2012, Vol. 39 ›› Issue (4): 647-654.

• 果树 • 上一篇    下一篇

36 份枣品种SSR 指纹图谱的构建

麻丽颖1,孔德仓2,刘华波1,王斯琪1,李颖岳1,庞晓明   

  1. (1 北京林业大学计算生物学中心,林木育种国家工程实验室,林木、花卉遗传育种教育部重点实验室,北京 100083;2 河北省沧县枣树国家良种基地,河北沧州 061000)
  • 出版日期:2012-04-25 发布日期:2012-04-25

Construction of SSR Fingerprint on 36 Chinese Jujube Cultivars

MA Li-ying1,KONG De-cang2,LIU Hua-bo1,WANG Si-qi1,LI Ying-yue1,and PANG Xiao-ming1,*   

  1. (1Center for Computational Biology,National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,Beijing Forestry University,Beijing 100083, China;2National Improved Cultivar Station of Jujube,Cangzhou,Hebei 061000,China)
  • Online:2012-04-25 Published:2012-04-25

摘要: 利用12 对SSR 引物对36 个枣品种进行分析,采用荧光M13 毛细管电泳技术进行多态检测,共检测到99 个多态位点,每对引物的多态位点数达到8.25,PIC 值变幅为0.62 ~ 0.85,平均为0.75。依据12 对SSR 引物在36 个品种中扩增的特异带型组合,采用引物—带型组合法构建了36 个枣品种的指纹图谱,并对这36 份枣品种做聚类分析,遗传相似系数在0.6667 ~ 0.9444 之间。研究结果为枣树分类、种质鉴定和分子育种提供了重要的工具。

关键词: 枣, SSR, 指纹图谱, 毛细管电泳, 遗传相似性

Abstract: In this study,36 Chinese jujube cultivars were analyzed by capillary electrophoresis using fluorescent M13 multi-state detection method with 12 pairs of newly developed SSR primers. Totally,99 polymorphic alleles were revealed,with an average of 8.25 for each primer pairs. Polymorphism information content values for the primer pairs ranged from 0.62–0.85,with an average of 0.75. A strategy of combining primer pair with distinct alleles for fingerprint construction was developed and applied to the 36 cultivars. The genetic similarities among cultivars range from 0.6667 to 0.9444,on which a phenetic
tree showing the relationship among the cultivars was constructed. The present results provide valuable tools for the cultivar classification,germplasm identification and molecular breeding of Chinese jujube.

Key words: jujube, SSR, fingerprint, capillary electrophoresis, genetic similarity

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