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园艺学报 ›› 2012, Vol. 39 ›› Issue (3): 588-592.

• 研究报告 • 上一篇    下一篇

亚洲百合与青岛百合杂交及其 FISH 快速鉴定

任贵玲, 张线线, 周树军   

  1. (浙江大学农业与生物技术学院园艺系,农业部园艺植物生长发育与品质调控重点开放实验室,杭州 310058)
  • 出版日期:2012-03-25 发布日期:2012-03-25

Hybridization and Identification Between Asiatic Lily and Lilium tsingtauense Using FISH

 REN  Gui-Ling, ZHANG  Xian-Xian, ZHOU  Shu-Jun   

  1. (The Key Laboratory of Horticultural Plant Growth,Development and Quality Improvement,the Ministry of Agriculture of the People’s Republic of China;Department of Horticulture,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China)
  • Online:2012-03-25 Published:2012-03-25

摘要: 利用切柱头授粉和胚抢救技术获得亚洲百合‘Petit Brigitte’× 青岛百合的14 个杂交后代,以45S rDNA 为探针对亲本和其中4 个后代进行了荧光原位杂交(FISH)分析,结果表明,亲本和后代都为二倍体(2n = 2x = 24);‘Petit Brigitte’有10 个45S rDNA 位点,分别位于1、2、4、5 和7 号染色体上;青岛百合有8 个45S rDNA 位点,分别位于3、4、5 和10 号染色体上;4 个杂种后代有9 个45S rDNA位点,分别源于‘Petit Brigitte’和青岛百合。

关键词: 百合, 远缘杂交, rDNA, 荧光原位杂交

Abstract: Hybridization between Asiatic lily‘Petit Brigitte’× Lilium tsingtauense was conducted with cut style pollination and embryo rescue. Fourteen progenies were obtained,and the parents and 4 progenies were analyzed using FISH with 45S rDNA as probe. The results showed that all of them are diploid(2n = 2x = 24);‘Petit Brigitte’has 10 loci of 45S rDNA distributed on chromosome CH-1,CH-2,CH-5 and CH-7 and Lilium tsingtauense has 8 on CH-3,CH-4,CH-5 and CH-10 respectively,2n = 2x = 24 for Lilium tsingtauense and 2n = 2x = 24 for hybrids,respectively;all four progenies have 9 loci of 45S rDNA,unambiguously,coming from‘Petit Brigitte’and Lilium tsingtauense respectively.

Key words: Lilium, distant hybridization, rDNA, fluorescence in situ hybridization(FISH)

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