https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2012, Vol. 39 ›› Issue (3): 561-566.

• 研究报告 • 上一篇    下一篇

大白菜单体的鉴定及其染色体行为研究

罗双霞,陈雪平,申书兴*   

  1. 河北农业大学园艺学院,河北保定 071001
  • 收稿日期:2011-12-27 修回日期:2012-02-20 出版日期:2012-03-25 发布日期:2012-03-25
  • 通讯作者: 申书兴 E-mail:shensx@hebau.edu.cn

Identification of Several Monosomics in Chinese Cabbage and Observation of Their Chromosome Behaviors

LUO Shuang-xia,CHEN Xue-ping,and SHEN Shu-xing*   

  1. College of Horticulture,Hebei Agricultural University,Baoding,Heibei 071001,China
  • Received:2011-12-27 Revised:2012-02-20 Online:2012-03-25 Published:2012-03-25
  • Contact: SHEN Shu-xing E-mail:shensx@hebau.edu.cn

摘要: 以同源四倍体大白菜小孢子植株为材料,采用根尖染色体数鉴定的方法,从534个小孢子植株中筛选出9株2n–1 = 19的植株,经核型分析和离体繁殖初步获得了3号、5号和6号3个单体。3个单体生殖生长期植株和花蕾形态及花粉特征与二倍体差别明显,各单体间花蕾形态差异明显。在减数分裂终变期或中期Ⅰ,单体染色体主要以单价体的形式存在,后期Ⅰ染色体以9-10分离为主。

关键词: 大白菜, 染色体, 单体

Abstract: In the present paper,nine 2n–1 = 19 plants were obtained from the 534 microspore plantlets of autotetraploid Chinese cabbage by chromosome number examination,and then three monosomic lines(mono-3,mono-5 and mono-6)were preliminary identified by karyotype analysis and propagation in vitro. The plant and flower bud morphological characters,pollen viability and regularity were significantly different between the three monosomics and diploid. Flower bud type among the three monosomics also occurred markedly difference. The unitary chromosome of the monosomics was mainly existed in an univalent,occasionally formed a trivalent with another pair of homological chromosomes at diakinesis or at metaphaseⅠ,and the chromosome distribution of the monosomics was mainly 9-10 at anaphaseⅠ.

Key words: Chinese cabbage, chromosome, monosomic