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园艺学报 ›› 2012, Vol. 39 ›› Issue (3): 453-460.

• 果树 • 上一篇    下一篇

中国原产果梅自交亲和变异品种花粉决定基因SFB的插入突变

王培培,侍  婷,高志红*,章  镇*,庄维兵   

  1. 南京农业大学园艺学院,南京 210095
  • 收稿日期:2011-12-03 修回日期:2012-02-20 出版日期:2012-03-25 发布日期:2012-03-25
  • 通讯作者: 高志红,章 镇 E-mail:gaozhihong@njau.edu.cn,zhangzh@njau.edu.cn

Insertion Mutation of Pollen SFB Gene in Self-compatibility of Japanese Apricot Cultivars Native to China

WANG Pei-pei,SHI Ting,GAO Zhi-hong*,ZHANG Zhen*,and ZHUANG Wei-bing   

  1. College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China
  • Received:2011-12-03 Revised:2012-02-20 Online:2012-03-25 Published:2012-03-25
  • Contact: GAO Zhi-hong,ZHANG Zhen E-mail:gaozhihong@njau.edu.cn,zhangzh@njau.edu.cn

摘要: 果梅是配子体自交不亲和树种,但在长期进化过程中也出现了自交亲和品种。以果梅典型的自交不亲和品种‘南高’和自交亲和品种‘甲州小梅’为对照,采用田间自交授粉试验对原产于中国的‘四川白梅’和‘长农17’两个果梅品种进行了自交亲和性鉴定,结果表明:其自花授粉坐果率分别为30.73%和16.27%,属于自交亲和品种。进一步通过AS-PCR分析发现花粉SFB基因存在一段插入序列,造成了分子水平的基因突变。推测该基因的突变使‘四川白梅’和‘长农17’SFB功能发生改变,从而自交亲和。

关键词: 果梅, 自交亲和性, 鉴定, AS-PCR

Abstract: Japanese apricot(Prunus mume Sieb. et Zucc)is a fruit crop with gametophytic self-incompatibility. Some self-compatible varieties appeared during long-term evolution. In this study,a typical self-incompatibility cultivar‘Nanko’and self-compatible cultivar‘Koshinoume’of Japanese apricot were taken as controls. We identified the self-compatibility of two cultivars‘Sichuan Baimei’and ‘Changnong 17’native to China through field self-pollination test,the results demonstrated that the rate of fruiting set were 30.73% and 16.27%,respectively. Further analysis of AS-PCR showed that there were insertion mutation in pollen SFB genes of the two cultivars,which possibly resulted in self-compatibility of these two cultivars.

Key words: Japanese apricot, self-compatibility, identification, AS-PCR