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园艺学报 ›› 2012, Vol. 39 ›› Issue (2): 273-280.

• 蔬菜 • 上一篇    下一篇

钙素对SA诱导番茄幼苗抗灰霉病的调控作用

李琳琳,李天来*,余朝阁,张抗抗   

  1. 沈阳农业大学园艺学院,设施园艺省部共建教育部重点实验室,辽宁省设施园艺重点实验室,沈阳 110866
  • 收稿日期:2011-09-02 修回日期:2011-12-02 出版日期:2012-02-25 发布日期:2012-02-25
  • 通讯作者: 李天来

The Effect of Calcium on Regulation of SA-induced Resistance to Botrytis cinerea in Tomato

LI Lin-lin,LI Tian-lai*,YU Chao-ge,and ZHANG Kang-kang   

  1. College of Horticulture,Shenyang Agricultural University,Key Laboratory of Protected Horticulture Liaoning Provincial,Key Laboratory of Protected Horticulture,Ministry of Education,Shenyang 110866,China
  • Received:2011-09-02 Revised:2011-12-02 Online:2012-02-25 Published:2012-02-25
  • Contact: LI Tian-lai

摘要: 为探索钙对水杨酸(SA)诱导番茄(Solanum lycopersicum)幼苗抗灰霉病的作用,采用‘L402’番茄品种,通过分别喷施8 mmol • L-1 CaCl2和5 mmol • L-1 EGTA后再喷施2 mmol • L-1 SA,3 d后接种灰霉病菌孢子的方法,研究了不同处理对番茄幼苗灰霉病病情指数、活性氧积累、主要防御酶活性及其基因表达的影响。结果表明:接种灰霉病菌孢子5 d后,SA处理的番茄幼苗病情指数比对照降低37.27%,Ca + SA处理较SA处理进一步降低18%;接种1 d和2 d后,叶片中产生速率和H2O2含量分别出现应激高峰,且处理间存在差异,与对照相比Ca + SA处理分别提高33.05%和29.31%,EGTA + SA处理分别降低32.62%和46.34%;叶片中抗病相关酶活性和基因表达在接种病菌后也出现应激高峰,其中Ca + SA处理显著提高了PAL、几丁质酶、β–1,3–葡聚糖酶活性,EGTA处理及EGTA + SA处理显著降低了PAL、几丁质酶、β–1,3–葡聚糖酶活性。上述结果表明,Ca2+在SA诱导番茄抗灰霉病中具有正调控作用,而且这种作用与PAL、几丁质酶、β–1,3–葡聚糖酶活性及其基因表达密切相关。

关键词: 番茄, 灰霉病, 钙, 水杨酸, 诱导抗病性, 调控

Abstract: In order to explore the effect of calcium on the SA-induced resistance to Botrytis cinerea in tomato(Solanum lycopersicum). Tomato Botrytis cinerea susceptible strain‘L402’was pretreated with foliar spraying 8 mmol • L-1 CaCl2 and 5 mmol • L-1 EGTA,respectively. Then followed by application of exogenous 2 mmol • L-1 SA,3 d before B. cinerea infection. The disease index,ROS accumulation,variations of the activities of main resistance enzymes and their gene expression were analyzed. The results show that the disease index is significantly reduced by 37.27% compared to control in SA treatment 5 d after B. cinerea infection;While in Ca + SA treatment,the disease index reduced by 18% compared to SA treatment. The  production rate and H2O2 content reached the highest level at 1 d and 2 d,respectively,there is difference among treatments:production rate of  and H2O2 content in tomatoes treated by Ca + SA are 33.05% and 29.31% higher than control(H2O),however,under EGTA + SA treatment significantly reduced by 32.62% and 46.34%,respectively. The peak of disease defense-related enzymes activity and gene expression also exist,difference obtained among treatments:PAL,chintase and β-1,3-glucancse treated by Ca + SA are significantly higher than control,whereas EGTA and EGTA + SA significantly suppress PAL,chitinase,β-1,3-glucanase activity. These results indicate that Ca2+ plays a positive regulatory role in the SA-induced resistance to Botrytis cinerea in tomato,which is closely related to the PAL,chitinase,β-1,3-glucanase activity and gene expression.

Key words: tomato, Botrytis cinerea, calcium, salicylic acid, induce resistance, regulation

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