多倍体无籽罗汉果及其亲本遗传背景的ISSR分析

园艺学报 ›› 2012, Vol. 39 ›› Issue (2): 387-394.

多倍体无籽罗汉果及其亲本遗传背景的ISSR分析

韦荣昌^1,2,3，李虹¹，蒋建刚⁴，李锋^1,5，蒋水元¹，黄夕洋^1,*

1广西壮族自治区中国科学院广西植物研究所，广西桂林 541006；2中国医学科学院 & 北京协和医学院，药用植物研究所，北京 100193；3广西药用植物园，南宁 530023；4桂林亦元生现代生物技术有限公司，广西桂林 541004；5广西科学院，南宁 530003

收稿日期:2011-09-07 修回日期:2011-12-27 出版日期:2012-02-25 发布日期:2012-02-25
通讯作者: 黄夕洋 E-mail:xiyang0687@163.com

ISSR Analysis of Genetic Background of Polyploidy Seedless Siraitia grosvenorii and Their Parents

WEI Rong-chang^1,2,3，LI Hong¹，JIANG Jian-gang⁴，LI Feng^1,5，JIANG Shui-yuan¹，and HUANG Xi-yang^1,*

1Guangxi Institute of Botany，Guangxi Zhuangzu Autonomous Region and Chinese Academy of Sciences，Guilin，Guangxi 541006，China；2Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences & Peking Union Medical College，Beijing 100193，China；3Guangxi Botanical Garden of Medicinal Plants，Nanning 530023，China；4Guilin Sunnylife Modern Bio-Tech INC，Guilin，Guangxi 541004，China；5Guangxi Academy of Sciences，Nanning 530003，China

Received:2011-09-07 Revised:2011-12-27 Online:2012-02-25 Published:2012-02-25
Contact: HUANG Xi-yang E-mail:xiyang0687@163.com

摘要/Abstract

摘要： 应用ISSR分子标记探讨多倍体无籽罗汉果及其亲本材料的遗传背景。结果发现：多倍体无籽罗汉果及其亲本的相似性系数为0.6399 ~ 0.8566，F₁代与四倍体母本之间的平均相似性系数高于其与二倍体父本之间的相似性系数，说明子代从母本继承的遗传物质更多，遗传上倾向母本。且F₁代与亲本之间的平均遗传相似性系数大于或小于亲本之间，随亲本的组合和相应的F₁代而定；结合聚类图和双变量主坐标分析，可知：基本上子代和母本排列并聚在一起，而且父本、母本、子代彼此之间距离相对较近地聚在一起；因此还是体现了“子似亲”的遗传现象。

关键词: 罗汉果, 多倍体, 遗传背景, ISSR

Abstract: In this study，the genetic background of polyploidy seedless Siraitia grosvenorii and their parents were revealed by ISSR（Inter simple sequence repeats）molecular marker technique. The results showed that the genetic similarity coefficient between two individuals was from 0.6399 to 0.8566，and the average coefficient between F₁generation and tetraploid female parent was higher than that between F₁generation and diploid male parent，which showed that the F₁ generation got more genetic substance from female parent than that from male parent，and the genetic phenomenon showed F₁generation was apt to female parent. And the average coefficient between F₁generation and parents was higher or lower than that between parents. According to dendrogram analysis and double principal coordinate analysis，filial generation and female parent arrayed and clustered together，and if genetic distance of male parent，female parent and filial generation was near，they would array together too. So，in general，the genetic phenomenon embodied“filial generation was similar to parents”.

Key words: Siraitia grosvenorii, polyploidy, genetic background, ISSR

中图分类号:

S 668.9

韦荣昌, 李虹, 蒋建刚, 李锋, 蒋水元, 黄夕洋. 多倍体无籽罗汉果及其亲本遗传背景的ISSR分析[J]. 园艺学报, 2012, 39(2): 387-394.

WEI Rong-Chang, LI Hong, JIANG Jian-Gang, LI Feng, JIANG Shui-Yuan, HUANG Xi-Yang. ISSR Analysis of Genetic Background of Polyploidy Seedless Siraitia grosvenorii and Their Parents[J]. ACTA HORTICULTURAE SINICA, 2012, 39(2): 387-394.

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