关键词: 梨, 花柱, 内源激素, 矿质元素, 原位萌发

Abstract: ‘Dangshan Suli’and‘Housi’pear were as experiment materials in this study，the hormones and mineral elements content in pollen，style and ovary were respectively measured by method of ELISA （Enzyme-Linked Immunosorbent Assay）and ICP-AES（Inductively Coupled Plasma Atomic Emission Spectrometry），the effects of the hormone and mineral elements levels in style on pollen germination were discussed. The results showed that hormone levels in style exhibit gradient distribution，from the top to the bottom in style morphology，the endogenous hormones ABA，IAA，ZR contents were gradually increased，while GA₃ was gradually decreased；The mineral elements K，Ca，Fe，Zn，B showed a gradually decreased. The elongation of pollen tube could be promoted treated with 2.5 mg · L^-1 IAA and 5 mg · L^-1 6-BA；Further analysis showed that the ABA，IAA contents in style is higher than that in pollen，so the major hormones for pollen germination requirement probably come from style，because the elongation of pollen tubes could be promoted by increased hormone levels in style. The mineral elements K，Ca，Fe，Zn and B in pollen higher than the style and ovary，therefore，the required mineral elements in the process of pollen germination may come from the pollen itself，because the elongation of pollen tubes could not be significantly promoted by increased mineral levels in style.

Key words: pear, style, endogenous hormone, mineral element, in situ germination