关键词: 羽衣甘蓝, Exo70A1, 序列分析, 表达分析

Abstract: The Exo70A1 gene（BoExo70A1）was isolated from stigma of ornamental kale（Brassica oleracea var. acephala）S13-b homozygotes，whose GenBank accession number is JF919716. BoExo70A1 cDNA was 2 184 bp in the full length，which contained a 5′-untranslated region（5′-UTR）of 56 bp，a 3′-UTR of 211 bp，and an opening reading frame of 1 917 bp encoding a 638 predicted amino acids residue protein. The deduced amino acid sequence of BoExo70A1 shared 99% and 94% identity with Brassica napus Exo70A1 and Arabidopsis thaliana Exo70A1，respectively. The genomic structure of BoExo70A1 contained 12 exons and 11 introns with boundary sequences of GT at 5′ site and AG at 3′ site，which complied with the typical‘GU-AG rule’in plants. Southern hybridization analysis showed that there were multiple copies of BoExo70A1 gene in genome. Northern hybridization analysis showed that the BoExo70A1 was ubiquitous expressed in stem，petal，anther，stigma，style and ovary，as well as a relative low expression in leaf.

Key words: Brassica oleracea var. acephala, Exo70A1, sequence analysis, expression analysis