关键词: 月季, 切花, 花瓣扩展, 乙烯, Rh-ADF1, 表达调节, 亚细胞定位

Abstract: In the present work，we isolated an ADF gene（actin depolymerizing factor）from petals of roses（Rosa hybrid‘Samantha’）and named it as Rh-ADF1 with accession NO. JN048105 and deposited in GenBank datebase. The cDNA full length of Rh-ADF1 contains an open reading frame with 423 bp in length，encoding a protein of 140 amino acids. Semi-quantitative RT-PCR analysis showed that expression level of Rh-ADF1 was slightly higher in petals than other four floral organs，including sepals，gynoecia，androecia，receptacles. The accumulation of Rh-ADF1 transcripts was strongly increased by ethylene in petals，and was obviously suppressed by 1-MCP，an action inhibitor of ethylene. Transient expression of GFP-Rh-ADF1 fusion gene in onion epidermal cells showed that Rh-ADF1 protein distributedubiquitously in the cell，while it preferentially located on filament networks of actins. Together，our results suggested that Rh-ADF1 may be involved in ethylene-regulated petal cell expansion at transcriptional level.

Key words: rose, cut flower, petal expansion, ethylene, Rh-ADF1, transcription regulation