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园艺学报 ›› 2012, Vol. 39 ›› Issue (1): 91-100.

• 蔬菜 • 上一篇    下一篇

拟南芥中异源过表达黄瓜CsTRY基因对表皮毛的抑制作用

谭 峥1,郭 芳1,杨福强2,刘丽英1,张小兰1,任华中1,*   

  1. (1中国农业大学农学与生物技术学院蔬菜系,北京100193;2湖南省长沙市蔬菜研究所,长沙410003)
  • 收稿日期:2011-10-26 修回日期:2011-12-24 出版日期:2012-01-25 发布日期:2012-01-25
  • 通讯作者: 任华中

Overexpression of Cucumber CsTRY Greatly Represses Trichome Formation in Arabidopsis

TAN Zheng1,GUO Fang1,YANG Fu-qiang2,LIU Li-ying1,ZHANG Xiao-lan1,and REN Hua-zhong1,*   

  1. (1College of Agronomy & Biotechnology,China Agricultural University,Beijing 100193,China;2Vegetable Science Institute of Changsha,Changsha 410003,China) 
  • Received:2011-10-26 Revised:2011-12-24 Online:2012-01-25 Published:2012-01-25
  • Contact: REN Hua-zhong

摘要: 将拟南芥表皮毛调控基因TRIPTYCHON(TRY)、CAPRICE(CPC)以及ENHANCER OF TRY AND CPC1(ETC1)的序列信息,在黄瓜基因组数据库中进行BLAST比对后发现,它们都对应于黄瓜中的同一个基因CsTRY。异源过量表达黄瓜CsTRY导致拟南芥叶片表皮毛大量减少,且使拟南芥中表皮毛起始基因GLABRA2(GL2)的表达量显著下降,表明黄瓜CsTRY基因与拟南芥TRY基因可能具有功能上的保守性,通过阻碍GL1-GL3/EGL3-TTG1三聚体复合物的形成来降低GL2的表达,从而抑制表皮毛的形成。

关键词: 黄瓜, 表皮毛, CsTRY, 转基因, 表达分析

Abstract: Using the sequence information of trichome regulating genes TRIPTYCHON(TRY),CAPRICE(CPC)and ENHANCER OF TRY AND CPC1(ETC1)in Arabidopsis to perform BLAST analysis against Cucumber Genome Database. We find that TRY,CPC and ETC1 all result in the same hit CsTRY in the cucumber genome. Overexpression of CsTRY in Arabidopsis greatly decrease the number of trichomes on the leaves,and significantly reduce the expression of trichome initiating gene GLABRA2(GL2),indicating that CsTRY may have the conserved function as Arabidopsis TRY,which inhibits trichome formation through reducing GL2 expression resulting from inhibition of the GL1-GL3/EGL3-TTG1 complex formation.

Key words: cucumber, trichome, CsTRY, transgene, expression analysis

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