关键词: 梨, 黑星病, remorin, 基因表达, 转基因烟草

Abstract: Apathogen-inducible expression gene fragment，from a previously constructed Suppression Subtractive Hybridization（SSH）cDNA library of‘Zaosuli’pear，was selected to obtain the full length of cDNA by RACE technique. The obtained cDNA，designated PbzsREMORIN，was 897 bp in length with a 597 bp open reading frame（ORF）and encoded 198 amino acid residues. Bioinformatics analysis indicated that PbzsREMORIN possesses a conserved domain of remorin family genes. The result from real-time RT-PCR assay revealed that the target gene was induced by Venturia nashicola. Moreover，the PbzsREMORIN gene was fused with GFP and transferred into onion epidermal cells via particle bombardment. Transient expression assay demonstrated that the PbzsREMORIN protein was located in the cell membrane，cytoplasm and nucleus. The PbzsREMORIN gene was transferred into Nicotiana tabacum ‘NC89’and totally obtained 27 transgenic lines. Analysis of in-vitro transgenic tobacco leaves with Ralstonia solanacearum suggested that overexpression of PbzsREMORIN gene in tobacco increased resistance to R. solanacearum，which indicated that the gene may play a critical role in plant-pathogen interaction.

Key words: pear, scab, remorin, gene expression, transgenic tobacco