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园艺学报 ›› 2012, Vol. 39 ›› Issue (1): 49-56.

• 果树 • 上一篇    下一篇

酚类物质对东北山樱生长及呼吸关键酶的影响  

吕德国1,李志霞1,2,秦嗣军1,*,马怀宇1   

  1. (1沈阳农业大学园艺学院,沈阳 110866;2中国农业科学院果树研究所,辽宁兴城 125100)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-01-25 发布日期:2012-01-25
  • 通讯作者: 秦嗣军

Effects of Phenolic Compounds on the Growth and Key Enzymes in Respiration of Cerasus sachalinensis

Lü De-guo1,LI Zhi-xia1,2,QIN Si-jun1,*,and MA Huai-yu1   

  1. (1College of Horticulture,Shenyang Agricultural University,Shenyang 110866,China;2China Research Institute of Pomology,Chinese Academy of Agricultural Sciences,Xingcheng,Liaoning 125100,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-01-25 Published:2012-01-25
  • Contact: QIN Si-jun

摘要: 以东北山樱(Cerasus sachalinensis Kom.)幼苗为试验材料,研究了根施酚类物质对羟基苯甲酸和香豆素对其生长和呼吸关键酶的影响。结果表明,低浓度的香豆素显著促进东北山樱幼苗生长,提高幼苗光系统Ⅱ(PSⅡ)最大光化学效率、呼吸底物含量、中间产物丙酮酸含量和苹果酸脱氢酶(MDH)活性,不影响糖酵解途径(EMP)和磷酸戊糖途径(PPP)关键酶活性;高浓度香豆素抑制生长,降低PSⅡ最大光化学效率、淀粉含量、异柠檬酸脱氢酶(IDH)和磷酸果糖激酶(PFK)活性。对羟基苯甲酸及其与香豆素的混合溶液在3个作用浓度(0.1、1.0和10 mmol · L-1)下均不同程度地抑制幼苗生长,降低柠檬酸含量,促进PPP关键酶活性提高。低浓度对羟基苯甲酸对幼苗PSⅡ最大光化学效率和呼吸底物含量无影响,但提高丙酮酸含量、己糖激酶(HK)、丙酮酸激酶(PK)和琥珀酸脱氢酶(SDH)活性,降低IDH、MDH活性;高浓度对羟基苯甲酸使幼苗PSⅡ最大光化学效率、呼吸底物和中间产物含量、PFK、PK、IDH、MDH活性下降,HK活性提高。

关键词: 东北山樱, 酚类物质, 呼吸, 生长, 关键酶

Abstract: Effects of two phenolic compounds(p-hydroxybenzoic acid and coumarin)on growth and pivotal respiratory indicators were studied using Cerasus sachalinensis Kom. seedlings as materials. The results showed that the growth of C. sachalinensis seedlings was highly promoted by low concentration of coumarin,and which also enhanced the PSⅡ maximum photochemical efficiency,respiratory substrate content,pyruvate content of metabolic intermediates and MDH activity. However,the key enzyme activities of EMP and PPP were not affected. Coumarin at high concentrations showed inhibitions to seedlings,while brought the PSⅡ maximum photochemical efficiency,starch contents,IDH activities and PFK activities down. p-hydroxybenzoic acid and the mixed solution of the two compounds at the 3 testing concentrations(0.1,1.0 and 10 mmol · L-1) both restrained seedling growth at certain extent. Contents of citric acid were reduced and key enzyme activities of PPP were improved. The PSⅡ maximum photochemical efficiency and respiratory substrate content were not changed by low concentration of p-hydroxybenzoic acid. However,pyruvate content,HK,PK,SDH activities were promoted. On the contrary,IDH,MDH activities were lowered. p-hydroxybenzoic acid at high concentrations showed suppressions on PSⅡmaximum photochemical efficiency,respiratory substrate contents,respiratory intermediates contents,and PFK,PK,IDH,MDH activities,but enhancement on HK activity.

Key words: Cerasus sachalinensis, phenolic compounds, respiration, growth, key enzyme

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