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园艺学报 ›› 2012, Vol. 39 ›› Issue (1): 1-12.

• 果树 •    下一篇

湖北海棠二价融合表达载体MhT2AC的构建及转化烟草耐盐性分析

张计育1,2,王三红1,乔玉山1,渠慎春1,郭忠仁2,*,章 镇1,*
  
    

  1. (1南京农业大学园艺学院,南京 210095;2江苏省中国科学院植物研究所,南京 210014)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-01-25 发布日期:2012-01-25
  • 通讯作者: 郭忠仁,章 镇

Construction of a Divalent Plant Binary Expression Vector MhT2AC from Malus hupehensis and Analysis of Salinity Tolerance in the Tobacco Transformants

ZHANG Ji-yu1,2,WANG San-hong1,QIAO Yu-shan1,QU Shen-chun1,GUO Zhong-ren2,*,and ZHANG Zhen1,*   

  1. (1College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;2Institute of Botany,Jiangsu Province and the Chinese Academy of Sciences,Nanjing 210014,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-01-25 Published:2012-01-25
  • Contact: GUO Zhong-ren,ZHANG Zhen

摘要: 利用口蹄疫病毒2A序列将湖北海棠MhTGA2转录因子和几丁质酶基因(Mhchitl)两个抗病相关基因融合在同一个开放阅读框下,构建二价融合植物双元表达载体,命名为MhT2AC。将该载体转化烟草,获得了8个转基因株系,MhTGA2基因和Mhchitl基因在烟草中共表达,并且转基因株系中NtSODNtAPXNtPPO等抗逆基因的表达量均加强;转基因烟草T1代植株抗盐性增强。

关键词: 湖北海棠, 二价融合表达载体, MhTGA2转录因子, 几丁质酶基因, 耐盐性

Abstract: The plant binary expression vector containing the divalent genes of MhTGA2 and Mhchitl isolated from Malus hupehensis and fused in a single open reading frame(ORF)by foot and mouth disease virus(FMDV)were constructed. The vector was then introduced into tobacco via Agrobacterium-mediated. The expression of NtSODNtAPX and NtPPO genes were detected and salinity tolerance was evaluated in the tobacco transformants. The results showed that we successfully constructed a plant binary expression vector with bivalent fusion and denoted it as MhT2AC. The MhTGA2 gene and the Mhchitl gene were co-expression in the transformants. Comparing with the controls,the expression of NtSODNtAPX and NtPPO genes were enhanced in transgenic lines. Moreover,the salinity tolerance of the T1 transformants were significantly improved.

Key words: Malus hupehensis, MhT2AC, MhTGA2, Mhchitl, salinity tolerance

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