机械伤促进红色砂梨着色的机制分析

园艺学报 ›› 2012, Vol. 39 ›› Issue (1): 23-30.

机械伤促进红色砂梨着色的机制分析

张东1，俞波1，钱敏杰1，王苏珂2，李秀根2，滕元文1,*

（1浙江大学园艺系，农业部园艺植物生长发育与品质调控重点开放实验室，杭州 310058；2中国农业科学院郑州果树研究所，郑州 450009）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-01-25 发布日期:2012-01-25
通讯作者: 滕元文

The Mechanism Analysis of Anthocyanin Accumulation in Red Chinese Sand Pears Enhanced by Wounded Treatments

ZHANG Dong1，YU Bo1，QIAN Min-jie1，WANG Su-ke2，LI Xiu-gen2，and TENG Yuan-wen1,*

（1Department of Horticulture，The State Agricultural Ministry Laboratory of Horticultural Plant Growth，Development & Quality Improvement，Zhejiang University，Hangzhou 310058，China；2Zhengzhou Fruit Institute，Chinese Academy of Agricultural Sciences，Zhengzhou 450009，China）

Received:1900-01-01 Revised:1900-01-01 Online:2012-01-25 Published:2012-01-25
Contact: TENG Yuan-wen

摘要/Abstract

摘要： 以红色砂梨（Pyrus pyrifolia Nakai）品种‘满天红’为试材，研究了采后机械伤处理对果实着色相关的生理指标和基因表达的影响。结果表明：机械伤处理能够有效促进果实受伤部位花色苷的积累，并伴随着该部位果实硬度显著下降，乙烯释放速率显著上升，花色苷合成相关基因PpF3H、PpANS、PpUFGT和PyMYB10表达量显著上调，而对照果实仅发生了轻微着色，且未检测到乙烯的释放。据此推测乙烯可能参与了红色砂梨果实花色苷合成的调控，对花色苷合成具有促进作用。机械伤处理可以作为一种有效的研究方法用于果实着色调控机制的研究。

关键词: 梨, 红色砂梨, 花色苷, 机械伤, 乙烯, 基因表达

Abstract: Effects of wounded treatments on post-harvest coloration and related physiology and gene expression in red Chinese sand pear（Pyrus pyrifolia Nakai）‘Mantianhong’were studied. Results showed that wounded treatments decreased fruit firmness，increased ethylene production rate of fruit，enhanced post-harvest anthocyanin accumulation in injured part of red Chinese sand pear，and up-regulated the transcript level of anthocyanin biosynthetic related genes：PpF3H，PpANS，PpUFGT and PyMYB10，markedly. Ethylene production of control fruit was under the detection limit，and only a trace amount of anthocyanin was detected in control fruit. Results above indicated that ethylene might be involved in the regulation of anthocyanin accumulation in red Chinese sand pear，and improve the level of anthocyanin accumulation in red Chinese sand pear. It was suggested that wounded treatments could be used as a mean to better understand the regulation mechanism of anthocyanin biosynthesis in fruits.

Key words: pear, red Chinese sand pear, anthocyanin, wounded, ethylene, gene expression

中图分类号:

S 661.2

张东;俞波;钱敏杰;王苏珂;李秀根;滕元文;. 机械伤促进红色砂梨着色的机制分析[J]. 园艺学报, 2012, 39(1): 23-30.

ZHANG Dong;YU Bo;QIAN Min-jie;WANG Su-ke;LI Xiu-gen;and TENG Yuan-wen;. The Mechanism Analysis of Anthocyanin Accumulation in Red Chinese Sand Pears Enhanced by Wounded Treatments[J]. ACTA HORTICULTURAE SINICA, 2012, 39(1): 23-30.

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