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园艺学报 ›› 2011, Vol. 38 ›› Issue (11): 2131-2139.

• 蔬菜 • 上一篇    下一篇

外源硫化氢对镉胁迫下黄瓜胚轴和胚根生理生化特性的影响

于立旭1,尚宏芹2,张存家1,王秀峰1,魏 珉1,杨凤娟1,史庆华1,*   

  1. (1山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安 271018;2菏泽学院生命科学系,山东菏泽 274015)
  • 收稿日期:2011-09-05 修回日期:2011-10-24 出版日期:2011-11-25 发布日期:2011-11-25
  • 通讯作者: 史庆华

Effects of Exogenous H2S on the Physiological and Biochemical Characteristics of the Cucumber Hypocotyls and Radicles under Cadmium Stress

YU Li-xu1,SHANG Hong-qin2, ZHANG Cun-jia1,WANG Xiu-feng1,WEI Min1,YANG Feng-juan1,and SHI Qing-hua1,*   

  1. (1State Key laboratory of Crop Biology,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018,China;2Department of Life Sciences,Heze University,Heze,Shandong 274015,China)
  • Received:2011-09-05 Revised:2011-10-24 Online:2011-11-25 Published:2011-11-25
  • Contact: SHI Qing-hua1

摘要: 以黄瓜(Cucumis sativus L.)种子为试材,研究了外源H2S预处理对镉胁迫下黄瓜胚轴和胚根生理生化特性的影响。结果表明:用浓度为300、600、900和1 200 μmol · L-1的H2S供体NaHS预处理黄瓜种子均能显著缓解镉胁迫对其胚轴和胚根的抑制作用,其中以900 μmol · L-1 NaHS处理效果最好。NaHS处理显著提高了镉胁迫下黄瓜子叶中淀粉酶的活性及胚轴与胚根中SOD、POD、CAT和APX活性,提高了二苯代苦味酰基自由基(DPPH· )清除能力和羟基自由基(OH· )清除能力,从而降低了MDA和H2O2含量,而其他钠盐(Na2S、Na2SO4、NaHSO4、Na2SO3、NaHSO3和NaAc)处理效果甚微,表明NaHS缓解镉胁迫对黄瓜胚轴和胚根生长的抑制作用归因于其释放出的H2S。

关键词: 黄瓜, 胚轴, 胚根, 硫化氢, 镉, 抗氧化酶

Abstract: The effects of exogenous H2S on physiological and biochemical characteristics of cucumber hypocotyls and radicles under cadmium stress were studied. The results showed that the inhibited growth of cucumber hypocotyls and radicles by cadmium was alleviated by different concentrations(300 μmol · L-1,600 μmol · L-1,900 μmol · L-1,1 200 μmol · L-1)of NaHS treatment,especially under the 900 μmol·L-1 treatment. NaHS treatment significantly increased activities of amylase,SOD,POD,CAT and APX as well as the capacity of α,α-diphenyl-β-picrylhydrazyl radical(DPPH· )and hydroxyl radical(OH· )scavenging in both hypocotyls and radicles of cucumber,as the results,the accumulations of MDA and H2O2 were decreased. Otherwise,the treatments with other sodium salt including Na2S,Na2SO4,NaHSO4,Na2SO3,NaHSO3 and NaAc had no significant effects on the growth of cucumber hypocotyls and radicles. Based on these results,it could be concluded that the function of NaHS was attributed to H2S from it.

Key words: cucumber, hypocotyl, radicle, hydrogen sulfide, cadmium, antioxidant enzyme

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