关键词: 寒兰, 杂交, 种子, 离体萌发, 繁殖

Abstract: The experiments were carried out for detecting fruit setting and in vitro seed germination in Cymbidium kanran. The results indicated that it was easy to cross between the lines of C. kanran，between C. kanran and C. goeringii，or C. sinense，according to the fact of 100% fruit setting. The percentage of fruit setting between C. kanran and hybrid Cymbidium depended on which species used as female parent. In the case of C. kanran as female parent，the fruit setting reached 75%，while hybrid Cymbidium as female parent the fruit setting dropped to zero. In the case of C. kanran as female parent and Phalaenopsis amabilis as male parent，no fruit was set. The fruits of C. kanran 180 to 240 d after pollination were the suitable materials for in vitro seed germination. The results also indicated that the optimum basal medium for seed in vitro germination was modified KC，the dark condition was necessary for germination，and the suitable combination of plant growth regulators for growth and differentiation of rhizomes was modified KC + 0.1 mg · L-1 6-BA + 5 mg · L-1 NAA.

Key words: Cymbidium kanran, hybridization, seed, in vitro germination, propagation