侵染水鬼蕉和花朱顶红的番茄斑萎病毒属病毒的电镜和DAS-ELISA诊断

园艺学报 ›› 2011, Vol. 38 ›› Issue (10): 2005-2009.

侵染水鬼蕉和花朱顶红的番茄斑萎病毒属病毒的电镜和DAS-ELISA诊断

方琦1,2，董家红1,2，丁铭1,2，尹跃艳2，张丽珍1,2，李婷婷1,2，苏晓霞1,2，李展1,2，张仲凯1,2,*

（1云南省农业科学院生物技术与种质资源研究所，昆明 650223；2云南省农业生物技术重点实验室，昆明 650223）

收稿日期:2011-07-25 修回日期:2011-10-08 出版日期:2011-10-25 发布日期:2011-10-25
通讯作者: 张仲凯1,2,*

DAS-ELISA and Electron Microscopic Diagnosis of Tospoviruses Infecting Hymenocallis littoraris and Hippeastrum vittatum in Kunming

FANG Qi1,2，DONG Jia-hong1,2，DING Ming1,2，YIN Yue-yan2，ZHANG Li-zhen1,2，LI Ting-ting1,2，SU Xiao-xia 1,2，LI Zhan 1,2，and ZHANG Zhong-kai1,2,*

（1Institute of Biotechnology and Germplasm Resources，Yunnan Academy of Agricultural Sciences，Kunming 650223，China；2Key Laboratory of Agricultural Biotechnology of Yunnan Province，Kunming 650223，China）

Received:2011-07-25 Revised:2011-10-08 Online:2011-10-25 Published:2011-10-25
Contact: ZHANG Zhong-kai1,2,*

摘要/Abstract

摘要： 从昆明市郊区采集到呈同心圆褪绿环斑、褐色枯斑症状的水鬼蕉［Hymenocallis littoraris （Jacq.）Salisb.］和花朱顶红［Hippeastrum vittatum（L’Hér.）Herb.］植株，采用电子显微镜技术和DAS-ELISA进行检测，在水鬼蕉和花朱顶红病叶分离物SGJ-KM和ZDH-KM的粗汁液和超薄切片中均观察到有类似番茄斑萎病毒属（Tospovirus）病毒粒体存在，该病毒粒子为球形，直径80 ~ 85 nm；病叶汁液与Tospovirus中的西瓜银色斑驳病毒（Watermelon silver mottle virus，WSMoV）/花生芽坏死病毒（Groundnut bud necrosis virus，GBNV）的复合抗血清呈阳性反应。通过形态学及血清学方法检测表明，侵染水鬼蕉和花朱顶红的病毒分离物SGJ-KM和ZDH-KM为Tospovirus的病毒分离物。

关键词: 水鬼蕉, 花朱顶红, 粒子形态, 番茄斑萎病毒属, DAS-ELISA

Abstract: The leaves of Hymenocallis littoraris（Jacq.）Salisb. and Hippeastrum vittatum（L’Hér.）Herb.，showing concentric chlorotic ring spot and brown lethal spot symptoms，were collected in Kunming. To confirm the presence of virus，the SGJ-KM and ZDH-KM infected leaves of H. littoraris and H. vittatum were detected by electron microscope observation and DAS-ELISA. Virions with a diameter of about 80–85 nm，similar in size to Tospovirus particles，were observed in the crude extracts and ultrathin sections of diseased leaves of H. littoraris and H. vittatum. The sap of diseased leaves had a positive reaction to the compound antibody of Watermelon silver mottle virus，WSMoV / Groundnut bud necrosis virus，GBNV. These results confirmed that H. littoraris and H. vittatum were infected by Tospoviruses.

Key words: Hymenocallis littoraris, Hippeastrum vittatum, morphology, Tospovirus, DAS-ELISA

中图分类号:

S 682

方琦;董家红;丁铭;尹跃艳;张丽珍;李婷婷;苏晓霞;李展;张仲凯;. 侵染水鬼蕉和花朱顶红的番茄斑萎病毒属病毒的电镜和DAS-ELISA诊断 [J]. 园艺学报, 2011, 38(10): 2005-2009.

FANG Qi;DONG Jia-hong;DING Ming;YIN Yue-yan;ZHANG Li-zhen;LI Ting-ting;SU Xiao-xia;LI Zhan;and ZHANG Zhong-kai;. DAS-ELISA and Electron Microscopic Diagnosis of Tospoviruses Infecting Hymenocallis littoraris and Hippeastrum vittatum in Kunming [J]. ACTA HORTICULTURAE SINICA, 2011, 38(10): 2005-2009.

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