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园艺学报 ›› 2011, Vol. 38 ›› Issue (10): 1991-1998.

• 研究报告 • 上一篇    下一篇

梨AFLP标记遗传图谱构建及果实相关性状的QTL定位

张瑞萍1,吴 俊1,李秀根2,*,杨 健2,王 龙2,王苏珂2,张绍铃1,*   

  1. (1南京农业大学梨工程技术研究中心,南京 210095;2中国农业科学院郑州果树研究所,郑州 450009)
  • 收稿日期:2011-05-30 修回日期:2011-10-09 出版日期:2011-10-25 发布日期:2011-10-25
  • 通讯作者: 李秀根2,*,张绍铃1,*

onstruction of AFLP Genetic Linkage Map and Analysis of QTLs Related to Fruit Traits in Pear

ZHANG Rui-ping1,WU Jun1, LI Xiu-gen2,*,YANG Jian2,WANG Long2,WANG Su-ke2,and ZHANG Shao-ling1,*   

  1. (1Center of Engineering and Technology Research,Nanjing Agricultural University,Nanjing 210095,China;2Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China)
  • Received:2011-05-30 Revised:2011-10-09 Online:2011-10-25 Published:2011-10-25
  • Contact: LI Xiu-gen2,*,ZHANG Shao-ling1,*

摘要: 以‘八月红’ב砀山酥梨’的97株F1群体为试材,利用AFLP技术构建梨(2n = 34)的分子遗传连锁图谱,共获得17个连锁群,包含209个标记位点,覆盖基因组1 506.3 cM,平均图距为7.21 cM;用区间作图法进行梨果实可溶性固形物、单果质量、果实纵径、果实横径和纵横径比等5个性状的QTL定位,其中在LG1连锁群上定位可溶性固形物含量QTL 1个;在LG6、LG7和LG11连锁群上定位单果质量QTL各1个;在LG7连锁群上定位果实横径的QTL1个;在LG6和LG7连锁群上定位果实纵径的QTL各1个;在LG1和LG8连锁群上定位果形指数的QTL各1个。各QTL位点的LOD值在2.50 ~ 4.14之间,可解释 11.4% ~ 36.4%的表型变异。

关键词: 梨, AFLP, 遗传图谱, 数量性状, 果实性状

Abstract: A genetic linkage map of Pyrus(2n = 34)was constructed by 209 AFLP markers which were distributed on 17 linkage groups and spanned 1 506.3 cM in genome with an average distance of 7.21 cM between markers. The map was based on a population(n = 97)derived from a cross of ‘Bayuehong’בDangshansuli’. By using interval mapping method,the QTLs for five fruit traits of pear were detected,including 1 QTL for soluble solids content on LG1,3 QTLs for fruit weight on LG6,LG7 and LG11,1 QTL for aspect ratio on LG7,2 QTLs for fruit weight on LG6 and LG7,and 2 QTLs for fruit stalk length on LG1 and LG8. The phenotypic variations explained by each QTL ranged from 11.4% to 36.4%,and their LOD values varied from 2.50 to 4.14.

Key words: Pyrus, AFLP, linkage map, QTL, fruit traits

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