关键词: 葡萄, VvGAI, 克隆, 表达, 亚细胞定位

Abstract: Full length cDNA sequence of potential GA response factor gene（VvGAI）was cloned from grapevine ‘Fujiminori’（Vitis vinifera × V. labrusca）using RT-PCR and rapid amplification of cDNA ends（RACE）. The sequence has been deposited in GenBank database with the accession number of HQ834311. Sequence analysis showed that the nucleotide sequence of this gene is 2 295 bp，containing a complete open reading frame that encodes 590 amino acids. Amino acid sequence analysis revealed that this potential VvGAI shared relatively high identity with the orthologs from Populus trichocarpa（68.56%），Arabidopsis thaliana（62.79%），Oryza sativa Indica Group（54.16%）. Semi-quantitative RT-PCR and fluorescent quantitative PCR analysis showed that VvGAI was expressed ubiquitously in vegetative and reproductive organs，such as shoot，leaf，flower and fruit. However，it was expressed the highest in shoot，which suggested that VvGAI could be a gene related to the rapid growth and division. Its expression in the developing fruits from treated inflorescence with GA solution was lower than that of thecontrol，about which the highest expression level was shown in young fruits. Subcellular localization assays showed that the VvGAI protein appeared in the nucleus.

Key words: grapevine, VvGAI, cloning, expression, subcellular localization