关键词: 红掌, 愈伤组织, 再生团块, 组织化学

Abstract:

Four kinds of callus from leaf and reproduction block mass(RBM) of aerial root in Anthurium andraeanum 'Arizona' were studied by slicing up and histochemistry coloration.The results showed that the saccharide was mainly grain-like in the callus and RBM.Protein was mainly in or around the nuclei. Saccharide was obviously surrounding the nuclei. In kelly callus from the outer layer of cells to the inner layer of cells saccharide and protein decreased as a visible grads, and in dark green callus the grads was unconspicuous. The saccharidein in the golden callus was very little without a grads. The saccharide distributed disperedly in the brown callus. There was a unconspicuous protein grads from the outer layer of cells to the inner layer of cells in the gold callus. However, there wasn't protein grads in the brown callus. There was also a visible grads of saccharide and protein when the RBM occurred，in the expending period of RBM there was not the grad. In the diffeneration stage of RBM the saccharide surrounded the dense small cell groups and the primary vascular tissue.The protein mainly concentrated in the nuclei of the cell groups. There were special cells in all classes of callus and RBM. The content and the grads of the saccharide was the important immanent condition for the diffenertiation of callus and RBM. The content of the protein and the integrality of the nuclei was very important for the diffenertiation of callus and RBM.

Key words: Anthurium andraeanum, callus, reproduction block mass, histochemistry