基于matK和rbcL DNA序列条形码鉴定柑橘及其近缘属植物

园艺学报 ›› 2011, Vol. 38 ›› Issue (09): 1733-1740.

基于matK和rbcL DNA序列条形码鉴定柑橘及其近缘属植物

于杰1，闫化学1，鲁振华3，周志钦1,2,*

（1南方山地园艺学教育部重点实验室，重庆 400715；2西南大学园艺园林学院，重庆 400716；3中国农业科学院郑州果树研究所，郑州 450009）

收稿日期:2011-04-18 修回日期:2011-08-23 出版日期:2011-09-25 发布日期:2011-09-25
通讯作者: 周志钦

dentifying Citrus and Its Related Genera Based on matK and rbcL DNA Sequence as Barcodes

YU Jie1，YAN Hua-xue1，LU Zhen-hua3，and ZHOU Zhi-qin1,2,*

（1Key Laboratory of Horticulture Science for Southern Mountainous Regions，Ministry of Education，Chongqing 400715，China；2College of Horticulture and Landscape Architecture，Southwest University，Chongqing 400716，China；3Zhengzhou Fruit Research Institute，Chinese Academy of Agricultural Sciences，Zhengzhou 450009，China）

Received:2011-04-18 Revised:2011-08-23 Online:2011-09-25 Published:2011-09-25
Contact: ZHOU Zhi-qin

摘要/Abstract

摘要： 对柑橘及其近缘属植物59份样品进行叶绿体matK和rbcL的序列测定，序列比对与人工校正，计算属间、种间以及种内的遗传距离，比较序列间的差异，构建系统发育树。结果表明，matK、rbcL及其组合（matK + rbcL）可以对柑橘及其近缘属属间进行鉴定，在种间水平上三者的鉴定率分别为55.9%、37.3%和83.0%。由此可见，与单一片段相比，matK + rbcL序列组合鉴定率更高，可用于对柑橘及其近缘属植物进行物种鉴定。

关键词: 柑橘属, 近缘属, matK, rbcL, DNA条形码

Abstract: The classification of Citrus is still a scientific problem unresolved because of apomixes，asexual variation，and interspecific hybridization in the genus Citrus. To use matK，rbcL，and them together to identify Citrus and its closely related genera，the chloroplast matK and rbcL sequences of 59 accessions were analyzed，the inter- and intraspecific genetic distances were calculated，and the phylogenetic trees of all the accessions tested were constructed based on the obtained distance data. The results indicated that matK and matK + rbcL could be successfully used to identify Citrus and its related genera. However，at the species level，matK，rbcL and their combination（matK + rbcL）only have correct identification frequency of 55.9%，37.3%，83.0% respectively. Compared with the single fragment，matK + rbcL was shown to be more powerful，implying that it can be used to identify the species of Citrus and its related genera.

Key words: Citrus, related genera, matK, rbcL, DNA barcode

中图分类号:

S 666

于杰;闫化学;鲁振华;周志钦;. 基于matK和rbcL DNA序列条形码鉴定柑橘及其近缘属植物[J]. 园艺学报, 2011, 38(09): 1733-1740.

YU Jie;YAN Hua-xue;LU Zhen-hua;and ZHOU Zhi-qin;. dentifying Citrus and Its Related Genera Based on matK and rbcL DNA Sequence as Barcodes[J]. ACTA HORTICULTURAE SINICA, 2011, 38(09): 1733-1740.

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