Abstract: Using the transgenic plant‘Fall Color’integrated AtDREB1A gene as the female parent，and the‘Asia Winter Light’，which has the better phenotype and wider application，as the male parent. Through the cross breeding method of non-emasculation artificial pollination，we obtained 158 filial generations. PCR and RT-PCR analysis of the part of progenies with superior ornamental mutation demonstrates that the exogenous gene can be transferred to the filial generations by sexual propagation and the AtDREB1A gene is over-expression in transgenic hybrids. Under low temperature，compared with the wild type，the growing of cuttage propogation plantlets of the transgenic hybrids which carrying exogenous DREB1A gene are much better，and the survival rates obviously increase under freezing stress. Physiological analysis demonstrates that the proline content and SOD activity of the transgenic hybrids are obviously raised under low temperature.

Key words: chrysanthemum, AtDREB1A gene, cross breeding, low temperature resistance, biophysical analysis