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园艺学报 ›› 2011, Vol. 38 ›› Issue (8): 1589-1594.

• 研究报告 • 上一篇    下一篇

中国石蒜属种间关系的trnH-psbA序列分析

全妙华1,2,3,*,欧立军1,2,3,佘朝文1,2,3,陈东明1,2,3,叶 威1   

  1. 1怀化学院生命科学系,湖南怀化 418008;2民族药用植物资源研究与利用湖南省重点实验室,湖南怀化 418008;3湘西药用植物与民族植物学湖南省高校重点实验室,湖南怀化 418008
  • 收稿日期:2010-12-01 修回日期:2011-06-30 出版日期:2011-08-25 发布日期:2011-08-25
  • 通讯作者: 全妙华1,2,3,*

Analysis of Interspecific Relationships in Lycoris Herb. Based on trnH-psbA Sequence

QUAN Miao-hua1,2,3,*,OU Li-jun1,2,3,SHE Chao-wen1,2,3,CHEN Dong-ming1,2,3,and YE Wei1   

  1. 1Department of Life Sciences,Huaihua University,Huihua,Hunan 418008,China;2Key Laboratory of Hunan Province for Study and Utilization of Ethnic Medicinal Plant Resources,Huihua,Hunan 418008,China;3Key Laboratory of Hunan Higher Education for Hunan-Western Medicinal Plant and Ethnobotany,Huihua,Hunan 418008,China
  • Received:2010-12-01 Revised:2011-06-30 Online:2011-08-25 Published:2011-08-25
  • Contact: QUAN Miao-hua1,2,3,*

摘要: 利用叶绿体基因trnH-psbA序列对石蒜属16种(含1变种)的种间关系进行了分析。结果表明:所测物种的序列全长在546 ~ 641 bp,GC含量为35.86% ~ 36.41%,其核苷酸变异位点14个,信息位点5个,这些位点可以区分石蒜属物种;供试材料聚为3类,除中国石蒜、忽地笑和玫瑰石蒜的系统位置有所不同外,与经典分类基本一致。叶绿体基因trnH-psbA序列可以有效地鉴别石蒜属物种及分类,是一种有效分子标记。

关键词: 石蒜属, trnH-psbA序列, 序列变异, 种间关系, 中国

Abstract: The interspecific relationship of 16 species(including 1 variation)in Lycoris was analyzed using trnH-psbA sequences. The results showed that the length of trnH-psbA sequences of the species was 546–641 bp,GC content of them was 35.86%–36.41%. There were 14 variable sites and 5 information sites,which could be used to identify some species of Lycoris. The clustering showed that Lycoris could be classified into 3 clades. The classification was basically consistent with that of morphology except for L. chinensis,L. rosea and L. aurea. This paper suggested that trnH-psbA could be used as a good molecular marker to identify or classify plants of Lycoris.

Key words: Lycoris, trnH-psbA sequence, nucleotide variation, interspecific relationship, China

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