不同形态氮素营养对大白菜芝麻状斑点病发生的影响

园艺学报 ›› 2011, Vol. 38 ›› Issue (8): 1489-1497.

不同形态氮素营养对大白菜芝麻状斑点病发生的影响

郭莹1,2，杨晓云1,*，司朝光1，张淑霞1，张清霞1，王媛1

1青岛市农业科学研究院，山东青岛 266100；2青岛农业大学园林园艺学院，山东青岛 266109

收稿日期:2011-03-01 修回日期:2011-07-06 出版日期:2011-08-25 发布日期:2011-08-25
通讯作者: 杨晓云1,*

Effects of Different Ratios of NO3- -N︰NH4+ -N on Black Sesame Spot in Chinese Cabbage

GUO Ying1,2，YANG Xiao-yun1,*，SI Chao-guang1，ZHANG Shu-xia1，ZHANG Qing-xia1，and WANG Yuan1

1Qingdao Academy of Agricultural Sciences，Qingdao，Shandong 266100，China；2College of Horticulture and Landscape，Qingdao Agricultural University，Qingdao，Shandong 266109，China

Received:2011-03-01 Revised:2011-07-06 Online:2011-08-25 Published:2011-08-25
Contact: YANG Xiao-yun1,*

摘要/Abstract

摘要： 选择高感“大白菜芝麻状斑点病”品系03B9大白菜为试材，调查不同形态氮素营养对其发病的影响；并选择高抗品系C24、P8-1和高感品系03B9、P8-2为试材，在总氮素浓度为25 mmol · L-1，NO3- -N︰NH4+ -N分别为3︰7、5︰5和7︰3的水培条件下，研究氮代谢和抗氧化系统生理指标的变化。结果表明：铵态氮促进芝麻状斑点病发生的作用高于硝态氮和酰胺态氮。随铵态氮比例的增大，感病品系03B9和P8-2较抗病品系病斑数显著增多，叶柄中PPO活性和铵态氮、MDA含量及电导率升高，NR活性和多酚含量降低；抗病品系C24和P8-1叶柄中GDH活性和多酚含量升高，PPO活性、MDA含量及电导率变化不明显。推测营养液中铵态氮的比例增加，影响感病品系氮素代谢中有关酶类的活性，使体内铵态氮过量积累，导致细胞膜系统受到伤害，使液泡内的酚类物质与细胞质中的多酚氧化酶接触，引起褐变，在叶柄表面表现出芝麻斑点症状。

关键词: 大白菜, 芝麻状斑点病, 氮素

Abstract: Chinese cabbage line 03B9 with high sensitivity to Black sesame spot were used as material to investigate the effects of (NH4)2SO4，NH4Cl，NH4NO3，urea，Ca(NO3)2 and KNO3 on Black sesame spot in Chinese cabbage. 03B9 and P8-2 with high sensitivity，C24 and P8-1 with high resistance to Black sesame spot were selected as materials，in hydroponic culture to study the effects of different NO3- -N︰NH4+ -N ratios（3︰7，5︰5，7︰3）with a total nitrogen concentration of 25 mmol · L-1 on nitrogen metabolism and antioxidant system. The determination indicators included activities of nitrate reductase （NR），glutamine synthetase（GS），glutamate dehydrogenase（GDH），polyphenoloxidase（PPO），the electric conductivity，and contents of nitrate nitrogen，ammonium nitrogen，malondialdehyde（MDA）and polyphenol. The results showed that ammonium nitrogen affected the occurrence of Black sesame spots more seriously than nitrate nitrogen and different NO3- -N︰NH4+ -N ratios affected the number of Black sesame spots. With the elevation of ammonium nitrogen ratio，the number of Black sesame spots in lines 03B9 and P8-2 increased more seriously than that in lines C24 and P8-1. In lines 03B9 and P8-2，the activity of PPO，electric conductivity，content of ammonium nitrogen and MDA increased，while the activity of NR and content of phenolics decreased. In lines C24 and P8-1，the activity of GDH and content of phenolics increased，while the activity of PPO，electric conductivity and content of MDA did not change significantly. It was presumed that in the high sensitive lines of Chinese cabbage，the increasing of the proportion of ammonium nitrogen influenced the activities of enzymes related to nitrogen metabolism，and then excessive ammonium nitrogen accumulated in plants，thus caused the injury of cell membrane. When contacted with PPO in cytoplasm phenolic compounds in vacuole were oxidized to quinone substances，and caused browning on the surface of petiole showing symptom of Black sesame spots. This research laid the foundation for further study on the mechanism of Black sesame spot in Chinese cabbage.　　

Key words: Brassica campestris L. ssp. pekinensis（Lour.）Olsson, Black sesame spot, nitrogen

中图分类号:

S 634.1

郭莹;杨晓云;司朝光;张淑霞;张清霞;王媛. 不同形态氮素营养对大白菜芝麻状斑点病发生的影响[J]. 园艺学报, 2011, 38(8): 1489-1497.

GUO Ying;YANG Xiao-yun;SI Chao-guang;ZHANG Shu-xia;ZHANG Qing-xia;and WANG Yuan. Effects of Different Ratios of NO3- -N︰NH4+ -N on Black Sesame Spot in Chinese Cabbage[J]. ACTA HORTICULTURAE SINICA, 2011, 38(8): 1489-1497.

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