关键词: 草莓, 果实, 脱落酸受体ABAR/CHLH, 基因, 表达, 蔗糖, pH

Abstract: To investigate the signalling perception mechanism of ABA regulation on strawberry fruit ripening，experiment with‘Beinongxiang’strawberry fruit during 1–4 weeks after anthesis，the mRNA expression level of FaABAR/CHLH gene in developing fruit and its affection factors were detected via real-time PCR and fruit-tissue discs. The results showed that a 4 325 bp cDNA fragment encoded a strawberry ABA receptor FaABAR/CHLH protein with 1 381 amino acid sequences that contain a conserved domain of metal ion chelatase H subunit superfamily. During the early developmental stage of fruit（small green，large green，and light green），the FaABAR/CHLH gene expressed highly. Coupled with fruit de-greening，the transcription level of the gene rapidly decreased，and then up to the lowest level at white stage. On the beginning of fruit red-coloring，the transcription level of the gene rapidly increased. Both ABA and pH could markedly promote the mRNA expression level of the gene. In contrast，sucrose significantly inhibited the gene transcripts. The change of the mRNA expression level of ABA receptor gene FaABA/CHLH during strawberry fruit development further uncovered an important role of ABA in regulation of fruit ripening.

Key words: strawberry, fruit, abscisic acid receptor ABAR/CHLH, gene, expression, sucrose, pH