https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
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https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
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https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2011, Vol. 38 ›› Issue (09): 1685-1692.

• 蔬菜 • 上一篇    下一篇

黄瓜无毛基因gl-2的遗传分析和定位

杨双娟1,苗 晗1,*,张圣平1,程周超1,周 健1,董邵云1,Todd C. Wehner2,顾兴芳1,**   

  1. 1中国农业科学院蔬菜花卉研究所,北京 100081;2美国北卡罗来纳州立大学园艺系,NC 27695,USA
  • 收稿日期:2011-06-16 修回日期:2011-08-23 出版日期:2011-09-25 发布日期:2011-09-25
  • 通讯作者: 顾兴芳

Genetic Analysis and Mapping of gl-2 Gene in Cucumber(Cucumis sativus L.)

YANG Shuang-juan1,MIAO Han1,*,ZHANG Sheng-ping1,CHENG Zhou-chao1,ZHOU Jian1,DONG Shao-yun1,Todd C. Wehner2,and GU Xing-fang1,**   

  1. 1Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2Department of Horticultural Science,North Carolina State University,Raleigh,NC 27695-7609,USA
  • Received:2011-06-16 Revised:2011-08-23 Online:2011-09-25 Published:2011-09-25
  • Contact: GU Xing-fang

摘要: 以黄瓜(Cucumis sativus L.)有毛类型‘9110Gt’(P1)和无毛突变体‘NCG-042’(P2)为试材,对无毛基因gl-2进行遗传分析和基因定位研究。结果表明,黄瓜的有毛、无毛性状由一对核基因控制,有毛对无毛为显性。结合分离群体分组混合分析法(bulked segregant analysis,BSA),以F2为作图群体,筛选得到18对与黄瓜无毛基因gl-2相关的SSR引物,构建了gl-2基因的SSR连锁群,并将该基因定位在黄瓜第2染色体上,两侧最近的连锁标记为SSR10522和SSR13275,遗传距离分别为0.6 cM和3.8 cM。经过回交群体验证,SSR10522和SSR13275的正确率分别为94.4%和91.6%。

关键词: 黄瓜, 无毛基因gl-2, 遗传分析, 基因定位, SSR

Abstract: Genetic analysis and gene mapping were carried out on gl-2 gene in cucumber(Cucumis sativus L.)using 9110Gt(with trichomes)and NCG-042(glabrous mutant)as experimental materials. The genetic analysis showed having trichomes or not is determined by a single nuclear gene,and the trait of having trichomes(Gl-2)is dominant to the glabrous(gl-2)in cucumber. Bulked segregant analysis(BSA)and simple sequence repeat(SSR)techonology were employed to mapping gl-2 gene of cucmber in F2 population. gl-2 gene was mapped to a linkage group with 11 SSR makers,corresponding to chromosome 2 of cucumber. The flanking markers SSR10522 and SSR132751 were linked to the gl-2 gene with genetic distances of 0.6 and 3.8 cM,respectively. The veracity of SSR10522 and SSR132751 was tested using BC1P2 population,and the accuracy rate for the two markers was 94.4% and 91.6%.

Key words: cucumber, gl-2 gene, genetic analysis, gene mapping, SSR marker

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