关键词: 板栗, 短雄花序, GID1, GA缺陷型突变体, 实时定量PCR

Abstract: GAs，CTK，ABA，Ethephon and IAA were used separately in feeding experiments，the result showed only the application of GAs can inhibit programmed cell death（PCD）in the top of short-male catkin and recover elongation growth to some extent. On the other hand，the partial cDNA sequnences encoding gibberellins receptor GID1 was isolated from the normal and mutant catkin of the Castanea mollissima by reverse transcription polymerse chain reaction（RT-PCR）. The results showed that the GID1 partial cDNA is 786 bp，putative amino acids have 91% similarity with GID1 in Populus trichocarpa and 85% in Ricinus communis and Gossypium hirsutum. According to the function analysis of putative animal acid sequnence，the GID1 is a functional GAs receptor gene in Castanea mollissima，and named CmGID1（accession numbers HQ651231）. Real-time PCR indicated that from primordia formation phase to early germina-tion of anther，the expressions of CmGID1 gene in mutant catkins are higher than that in normal catkins except the phase of May 23. Moreover，in the fast-growing phase，the expression of CmGID1 gene in mutant catkins are significantly higher than that in normal catkins（P < 0.01），while in growth recovered short male catkin treated after GAs，the expression of CmGID1 gene significantly decreased（P < 0.01）. In conclusion，it was preliminarily revealed that the short-catkin chestnut bud mutation is a GAs-deficient mutant.

Key words: Castanea mollissima, short male catkin, GID1, GAs-deficient mutant, Real-time quantitative PCR