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园艺学报 ›› 2011, Vol. 38 ›› Issue (7): 1325-1332.

• 蔬菜 • 上一篇    下一篇

甜椒疫病抗性遗传及相关基因分子标记研究

张晓芬,韩华丽,陈 斌,耿丽华,耿三省*   

  1. (北京市农林科学院蔬菜研究中心,北京100097)   
  • 收稿日期:2011-04-07 修回日期:2011-05-23 出版日期:2011-07-25 发布日期:2011-07-25
  • 通讯作者: 耿三省*

Genetic and Molecular Marker Analysis of Resistance to Phythophtora capsici in Sweet Pepper

ZHANG Xiao-fen,HAN Hua-li,CHEN Bin,GENG Li-hua,and GENG San-sheng*   

  1. (Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China)
  • Received:2011-04-07 Revised:2011-05-23 Online:2011-07-25 Published:2011-07-25
  • Contact: GENG San-sheng*

摘要: 以高抗疫病的甜椒育种材料‘N1345’与高感疫病辣椒材料‘N1308’为双亲,构建了6个联合世代,运用植物数量性状主基因 + 多基因联合分离分析方法对N1345疫病抗性进行遗传分析,并对其F2群体进行EST-SSR与SSR连锁标记分析。结果显示,N1345对疫病抗性由2对加性—显性—上位性主基因控制(B-1-1),两主基因加性效应、显性效应均相等,主基因遗传率在B1、B2和F2世代分别为63.43%、82.32%和83.46%。筛选到E73、E318等2个EST-SSR标记与疫病抗性病级均呈显著负相关,相关系数分别为﹣0.6296、﹣0.5492,差异水平均为极显著(P < 0.01),表明这2个标记与对疫病抗性基因紧密连锁。这2个标记在22份甜辣椒材料中分离一致,有6份材料抗、感表型与标记分离不一致。  

关键词: 甜椒, 疫病抗性, 遗传, EST-SSR

Abstract: Using the model of the major gene plus polygene of quantitative traits,a joint analysis of six-generations was performed from cross between a sweet pepper N1345 with high resistance to phythophtora blight and a highly susceptible hot pepper N1308. F2 population was analysed with EST-SSR and SSR molecular markers. It was showed that the resistance to Phythophtora blight of N1345 was controlled by two major genes(B-1-1)with equal additive and dominant effects. And the major genes heritabilities of B1,B2,F2 were 63.43%,82.32% and 83.46%,respectively. Two EST-SSR markers including E73 and E318 had significant minus correlation with desease indexes of Phythophtora capsici,with correlation coefficients–0.6296,–0.5492 separately(P < 0.01). It showed that the two markers were closely linked to genes of resistance to Phytophtora blight. Furthermore,the 2 EST-SSR markers had same speration in 22 pepper lines,and in 6 out 22 lines the resistance or susceptivity to Phythophtora wasn’t coincident with marker separation.

Key words: sweet pepper, resistance to Phythophtora blight, inheritance, EST-SSR

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