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园艺学报 ›› 2001, Vol. 28 ›› Issue (6): 544-550.

• 研究论文 • 上一篇    下一篇

茉莉幼苗ATP 酶活性的超微细胞化学定位与耐冷性研究

苏金为1;王湘平2   

  1. ( 1 福建农林大学基础部, 福州350002; 2 福建农林大学测试中心, 福州350002)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-12-25 发布日期:2001-12-25

Ultracytochemical Localization of ATP􀀁ase Activity in The Cells of Jas􀀁minum Sambac L. Seedlings and Its Relation to Chilling Tolerance

Su Jinwei1 and Wang Xiangping2   

  1. ( 1Department of Fundamental Subjects, Fuj ian Agriculture and Forestry University , Fuz hou 350002; 2Centre of Test, Fujian Agriculture and Forestry University , Fuzhou 350002)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-12-25 Published:2001-12-25

摘要: 采用磷酸铅沉淀的细胞化学方法, 电镜下观察两个抗冷力显著不同的茉莉品种幼苗ATP 酶活性在细胞中超微结构定位及其经冷胁迫的变化。结果表明, 抗寒力强的双瓣茉莉品种比抗寒力弱的单瓣品种在低温胁迫下超微结构和ATP 酶活性有更强的适应能力。茉莉幼苗叶肉组织ATP 酶主要定位于细胞质膜、液泡膜、叶绿体片层、线粒体脊和胞间连丝等部位, 小叶脉维管束的ATP 酶主要分布在筛管和伴胞。

关键词: 茉莉, 低温胁迫, 超微结构, ATP 酶

Abstract: Ultracytochmical localization of ATP􀀁ase act ivity was carried out using a leading phosphate precipitation technique in the cells of two different chilling resistance Jasminum sambac L. Ait. seedlings. Under 0 ! chilling stress, the ultrastructure of cells and the ATP􀀁ase activity in the chilling resistant Jasmine ( double petal cultivar) was obviously more stable than that in the chilling sensitive Jasmine ( single petal cult ivar) . The ATP􀀁ase in Jasmine seedlings cells of mesophyll mainly located on plasmolemma, tonoplast , chloroplast lamellae, mitochondria cristae, and plasmodeslma , whereas
the ATP􀀁ase in cells of minor leaf vein mainly located on the sieve element and the companion cell.

Key words: Jasmine ( Jasminum Sambac L. ), Low temperature stress, Ultrastructure, ATP􀀁, ase

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