原生质体非对称电融合获得不结球白菜胞质杂种

园艺学报 ›› 2001, Vol. 28 ›› Issue (6): 532-537.

原生质体非对称电融合获得不结球白菜胞质杂种

侯喜林¹；曹寿椿²；佘建明³；陆维忠³

( ¹ 南京农业大学作物遗传与种质创新国家重点实验室, 南京210095; ² 南京农业大学园艺学院, 南京 210095; ³江苏省农业科学院遗传生理所, 南京210014)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-12-25 发布日期:2001-12-25

Synthesis of Cytoplasm Hybrid of Non􀀁heading Chinese Cabbagethrough Asymmetric Electric Fusion of Protoplast Cell

Hou Xilin¹, Cao Shouchun², She Jianming³, and Lu Weizhong³

( ¹State Key Laboratory of Crop Genetics and Germplasm Enhancement , Nanj ing Agricultural University , Nanjing 210095; ²College of Horticulture, Nanj ing Agricultural University , Nanj ing 210095; ³I nstitute of Genetics and Physiology , Jiangsu Agricultural Academic Sciences , Nanjing 210014)

Received:1900-01-01 Revised:1900-01-01 Online:2001-12-25 Published:2001-12-25

摘要/Abstract

摘要： 以不结球白菜OguCMS‘91H 秋-100’ ( 不育系) 和‘91H 秋-1’( 保持系) 为试材, 研究了不同交流场强、交流频率、直流脉冲场强、直流幅宽及脉冲次数对非对称细胞融合效果的影响。结果表明, 不结球白菜电融合的最适电场条件为交流场强20 V/ cm、交流频率1500 kHz、直流场强200 V/ cm、直流脉冲幅宽40 􀀁s、直流脉冲次数3 次。融合产物用KM8P1培养基进行包埋培养, 获得了383 块小愈伤组织, 其中32 块分化出芽, 形成20 株完整植株,驯化后栽培成活8 株, ZS2、ZS6、ZS8 等3 株不育, 后用保持系花粉授粉, 分别收获种子。对胞质杂种的分子、细胞及生化分析将在其后代中继续进行。

关键词: 不结球白菜, 电融合, 再生植株, 胞质杂种

Abstract: The effects of different electric fields on the asymmetrical cell fusion of non􀀁heading Chinese cabbage was studied by using OguCMS 91Hqiu􀀁100 ( sterile line) and 91Hqiu􀀁21 (maintainer line) as materials. The best electric field for electric fusion was as follows: alternating current field intensity ( 20V/ cm) , alternating current frequency ( 1500 kHz) , direct current intensity ( 200 V/cm) , direct current pulse width ( 40 􀀁s) , direct pulse number ( 3 t imes) . The product of the fusion was embedded and cultured on KM8P1medium. 383 calli were obtained. Of these calli, 32 shoots differentiated and 20 plantlets developed. 8 plantlets were successfully transplanted after acclimatization.
Three of the plants were sterile. They set seeds after pollination with the maintainer pollen. Molecular, celluar and biochemistry analysis will be made on their progeny.

Key words: Non-heading Chinese cabbage, Electro􀀁, fusion, Regenerated plantlet, Cytoplasm hybrid

中图分类号:

S 634. 1

侯喜林;曹寿椿;佘建明;陆维忠. 原生质体非对称电融合获得不结球白菜胞质杂种[J]. 园艺学报, 2001, 28(6): 532-537.

Hou Xilin;Cao Shouchun;She Jianming;and Lu Weizhong. Synthesis of Cytoplasm Hybrid of Non􀀁heading Chinese Cabbagethrough Asymmetric Electric Fusion of Protoplast Cell[J]. ACTA HORTICULTURAE SINICA, 2001, 28(6): 532-537.

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