‘乌叶’荔枝胚胎发育过程特异蛋白的变化

园艺学报 ›› 2001, Vol. 28 ›› Issue (6): 504-508.

‘乌叶’荔枝胚胎发育过程特异蛋白的变化

陈　伟¹;吕柳新²;黄春梅¹;周　洁¹;梁文裕¹

(¹ 福建农林大学生命科学学院, 福州350002 ;　² 福建农林大学园艺学院, 福州350002)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-12-25 发布日期:2001-12-25

Studies on‘Wuye’Litchi Specific Proteins in the Embryo DevelopmentStages

Chen Wei1 , LüLiuxin2 , Huang Chunmei1 , Zhou Jie1 , and Liang Wenyu1

(¹College of Life Sciences , ² College of Horticultural Sciences , Fujian Agriculture and Fortestry University , Fuzhou 350002)

Received:1900-01-01 Revised:1900-01-01 Online:2001-12-25 Published:2001-12-25

摘要/Abstract

摘要： 应用改进的IEF2SDS2PAGE 技术比较分析了荔枝胚胎分化发育过程中蛋白质组分
的变化。结果表明, 大多数蛋白质组分在各发育时期的图谱相似, 但不同发育时期存在变化。
试验共发现了前期鱼雷胚的43. 7 kD、pI 3. 5 和55 kD、pI 9. 4 , 后期鱼雷胚的23. 4 kD、pI 6. 6和25. 1 kD、pI 6. 8 以及前期子叶胚的25. 1 kD、pI 9. 3 和64. 6 kD、pI 5. 9 等6 个新出现的特异蛋白。后期心形胚的图谱上蛋白质点数最多, 可分辨蛋白质斑点数约300 个, 这与蛋白质含量的测定结果一致。以上特异蛋白在多次重复中均显示出良好的重现性。因此认为, 这些特异蛋白在胚胎分化发育中可能起重要作用。

关键词: 荔枝, 胚胎, 特异蛋白, 分化发

Abstract: Modified IEF-SDS-PAGE technique was applied in comparing and analyzing the
changes of the protein components during the differentiation and development of litchi embryos. The results showed that while most of the proteins in different stages shared similar gel maps , which indicated
the stability of embryo protein components , some were found changing. This experiment discovered 6 new specific proteins in different stages of the embryo development . They were 43. 7 kD pI 3. 5 , 55. 0
kD pI 9. 4 in early torpedo-stages ; 23. 4 kD pI 6. 6 , 25. 1 kD pI 6. 8 in late torpedo2stage ; 25. 1 kD pI 9. 3 , 64. 6 kD pI 5. 9 in early cotyledon2stage. The number of the protein points was the highest in late heart2stage gel map , which indicated that gene expression was most active at the heart embryo stage. The number of protein spots visualized with silver staining is about 300. This result coincided with the one of the protein content determination. These specific proteins showed good repeatability in
the repeated experiments. It is therefore suggested that these specific proteins might play an important role in litchi embryo differentiation and development .

Key words: Litchi, Embryo, Specific protein, Embryo development

中图分类号:

S 667. 1

陈　伟;吕柳新;黄春梅;周　洁;梁文裕. ‘乌叶’荔枝胚胎发育过程特异蛋白的变化[J]. 园艺学报, 2001, 28(6): 504-508.

Chen Wei;LüLiuxin;Huang Chunmei;Zhou Jie;and Liang Wenyu. Studies on‘Wuye’Litchi Specific Proteins in the Embryo DevelopmentStages[J]. ACTA HORTICULTURAE SINICA, 2001, 28(6): 504-508.

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