百合的花药培养研究

园艺学报 ›› 2001, Vol. 28 ›› Issue (5): 472-474.

百合的花药培养研究

褚云霞^1，3；陈龙清^2，3*；黄燕文¹；张永春³

(¹ 华中农业大学理学系, 武汉430070 ;　² 华中农业大学林学系, 武汉430070 ;　³ 上海市农业科学院园艺研究所, 上海201106)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-10-25 发布日期:2001-10-25

Studies on Anther Culture of Lily

Chu Yunxia¹ , Chen Longqing² , Huang Yanwen¹ , and Zhang Yongchun³

(¹ Science department of Huazhong Agricultural University , Wuhan 430070; 　² Forestry department of Huazhong Agricultural University , Wuhan 430070;³ Horticultural Research Institute , Shanghai Academy of Agricultural Science ,Shanghai 201106)

Received:1900-01-01 Revised:1900-01-01 Online:2001-10-25 Published:2001-10-25

摘要/Abstract

摘要： 对百合花药培养的影响因素研究显示, 小孢子最适培养时期为单核期; 愈伤组织诱导率最高可达42. 16 % , 最佳激素组合为2 ,42D 3. 0 mg·L^-1+ KT 3. 0 mg·L^-1; 低温预处理作用不大; 早分化出的花蕾中的小孢子更易诱导产生愈伤组织; 分化培养基以改良MS + NAA(2. 0、4. 0 mg·L^-1) 为宜; 花粉植株中单倍体(n = 12) 约25 % , 二倍体(2 n = 24) 约75 %。

关键词: 百合, 花药, 组织培养

Abstract: The following experimental results have been obtained : Compared with other stage , microspores at uninucleate stage were more suitable for culture. Calli were induced from anthers of lily placed on modified MS media
supplemented with different concentrations of 2 ,4-dichlorophenoxyacetic acid (2 ,4-D) and Kinetin (KT) according to homogeneous design. The cold pretreatment (4 ℃, 2 days) couldn’t obviously raise the callus induction frequency in anther culture ; The pollen calli were transferred to modified MS media with different concentrations of naphthaleneacetic acid (NAA) . The results indicated that modified MS media with 2. 0 - 4. 0 mg·L^-1 NAA were optimal for the differentiation of pollen callus. Cytological examination of mitotic root tip cells from green plants showed that about 25 % of them were haploid and others were diploid.

Key words: Lily, Anther, Tissue culture

中图分类号:

S 682
Q 813

褚云霞;陈龙清;黄燕文;张永春. 百合的花药培养研究[J]. 园艺学报, 2001, 28(5): 472-474.

Chu Yunxia;Chen Longqing;Huang Yanwen;and Zhang Yongchun. Studies on Anther Culture of Lily[J]. ACTA HORTICULTURAE SINICA, 2001, 28(5): 472-474.

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