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园艺学报 ›› 2011, Vol. 38 ›› Issue (6): 1173-1179.

• 研究报告 • 上一篇    下一篇

低温胁迫对3个红掌品种叶片形态和生理特性的影响

田丹青1,2,葛亚英1,潘刚敏1,沈晓岚1,何 勇3,张 智1,刘晓静1,朱祝军2,3,*   

  1. (1浙江省萧山棉麻研究所,杭州 311202;2浙江大学农业与生物技术学院,杭州 310029;3浙江农林大学,浙江临安 311300)
  • 收稿日期:2011-02-21 修回日期:2011-05-31 出版日期:2011-06-25 发布日期:2011-06-25
  • 通讯作者: 朱祝军

Morphological and Physiological Characteristics of Different Cultivars of Anthurium andraenum Under Chilling Stress

TIAN Dan-qing1,2,GE Ya-ying1,PAN Gang-min1,SHEN Xiao-lan1,HE Yong3,ZHANG Zhi1,LIU Xiao-jing1,and ZHU Zhu-jun2,3,*   

  1. (1Zhengjiang Xiaoshan Cotton and Flax Research Institute,Hangzhou 311202,China;2College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310029,China;3Zhejiang Agriculture and Forestry University,Lin’an,Zhejiang 311300,China)
  • Received:2011-02-21 Revised:2011-05-31 Online:2011-06-25 Published:2011-06-25
  • Contact: ZHU Zhu-jun2

摘要: 以‘阿拉巴马’、‘大哥大’和‘粉冠军’3个红掌品种为试材,研究了低温胁迫对其叶片形态、叶片相对电导率(REC)、丙二醛(MDA)含量、过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶(SOD)活性的影响。结果表明:6 ℃低温胁迫48 h后,‘阿拉巴马’新叶颜色稍有变红,基本没有冷害症状;‘粉冠军’冷害表现最重,温度回升后叶片大量枯死,导致整株死亡;‘大哥大’冷害表现居中,新叶萎蔫。在低温胁迫下,‘阿拉巴马’电解质渗透率缓慢升高,MDA含量增加,CAT、POD和SOD活性均较高,且CAT活性持续上升,POD活性先下降后上升并趋于稳定,SOD活性较稳定,随着CAT活性的增强和POD、SOD酶活性的稳定,在低温胁迫36 h后,电解质渗透率和MDA含量开始下降;而‘大哥大’和‘粉冠军’在低温胁迫12 h后电解质渗透率和MDA含量明显增加,SOD活性开始下降,低温胁迫24 h后CAT活性开始下降,POD活性急剧上升。从形态和生理指标综合来说,3个品种的抗寒性以‘阿拉巴马’最强,‘大哥大’次之,‘粉冠军’最差。

关键词: 红掌, 低温胁迫, 相对电导率, MDA, CAT, POD, SOD

Abstract: Abstract:Three cultivars of Anthunium andraenum(‘Alabamb’,‘Dakote’and‘Pink Champion’)were used to investigate the effects of chilling stress on leaf morphology,relative electric conductivity(REC),malondialdehyde(MDA)content,and activities of catalase(CAT),peroxidases(POD)and superoxide dismutase(SOD). Results showed that after 48 h treatment under 6 ℃,‘Alabamb’ showed no symptoms of chilling injury except for slight red color with young leaves. However,‘Pink Champion’showed serious symptoms of chilling injury and then died after re-cultivated in greenhouse.‘Dakote’showed slight symptoms of chilling injury with the wilting of young leaves. Under chilling stress,REC and MDA content in‘Alabamb’leaves increased and activities of CAT,POD and SOD kept at higher level. CAT activity increased continuously with the treatment time,and POD activity decreased at the beginning and then increased to a high level,while SOD activity was relatively stable. After 36 h treatment of low temperature,REC and MDA content decreased. In the leaves of‘Dakote’and‘Pink Champion’,after 12 h treatment of low temperature,REC and MDA content increased obviously,and SOD activity began to decrease. CAT activity began to decrease,and POD activity increased sharply after 24 h treatment of low temperature. From the results of the present experiment,it was concluded that chilling resistance of 3 cultivars was decreased in the order of‘Alabamb’,‘Dakote’and‘Pink Champion’.

Key words: Anthunium andraenum, low temperature, relative electric conductivity, MDA, CAT, POD, SOD

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